Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering

In the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as poten...

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Authors: Mora Boza, Ana, López Ruiz, Elena, López Donaire, María Luisa, Jiménez, Gema, Rosa Aguilar, María, Marchal, Juan Antonio, Perdiz, Jose Luis, Vázquez Lasa, Blanca, San Román, Julio, Gálvez Martín, Patricia
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Universidad de Jaén
Repository:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
OAI Identifier:oai:ruja.ujaen.es:10953/2350
Online Access:https://hdl.handle.net/10953/2350
Access Level:Open access
Keyword:interpenetrated polymer network
semi-IPN
methacrylated hyaluronic acid
chitosan
glycerylphytate
mesenchymal stem cell
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spelling Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue EngineeringMora Boza, AnaLópez Ruiz, ElenaLópez Donaire, María LuisaJiménez, GemaRosa Aguilar, MaríaMarchal, Juan AntonioPerdiz, Jose LuisVázquez Lasa, BlancaSan Román, JulioGálvez Martín, Patriciainterpenetrated polymer networksemi-IPNmethacrylated hyaluronic acidchitosanglycerylphytatemesenchymal stem cellIn the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant di erences between them regarding composition, morphology, and mechanical properties after physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN membranes. The biological performance was evaluated on primary human mesenchymal stem cells (hMSCs) and the systems revealed no cytotoxic e ect. The excellent biocompatibility of the systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces. Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN, which suggested that these polymeric membranes could be proposed as an e ective promoter system of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes can provide a stable and amenable environment for hMSCs support and growth with potential applications in the biomedical field.The authors thanks to “La Caixa” Foundation (ID 100010434), which supported Ana Mora-Boza (scholarship code LCF/BQ/ES16/11570018) and to the Spanish Ministry of Economy and Competitiveness for financial support (project RTC-2016-5451-1) and the Fundación Mutua Madrileña (project FMM-AP17196-2019). M. R. Aguilar and B. Vázquez-Lasa are members of the SusPlast platform (Interdisciplinary Platform for Sustainable Plastics towards a Circular Economy) from the Spanish National Research Council (CSIC).MDPI202420242020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/10953/2350reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaéninstname:Universidad de JaénInglésPolymersAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ruja.ujaen.es:10953/23502026-06-24T12:41:07Z
dc.title.none.fl_str_mv Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
title Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
spellingShingle Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
Mora Boza, Ana
interpenetrated polymer network
semi-IPN
methacrylated hyaluronic acid
chitosan
glycerylphytate
mesenchymal stem cell
title_short Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
title_full Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
title_fullStr Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
title_full_unstemmed Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
title_sort Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
dc.creator.none.fl_str_mv Mora Boza, Ana
López Ruiz, Elena
López Donaire, María Luisa
Jiménez, Gema
Rosa Aguilar, María
Marchal, Juan Antonio
Perdiz, Jose Luis
Vázquez Lasa, Blanca
San Román, Julio
Gálvez Martín, Patricia
author Mora Boza, Ana
author_facet Mora Boza, Ana
López Ruiz, Elena
López Donaire, María Luisa
Jiménez, Gema
Rosa Aguilar, María
Marchal, Juan Antonio
Perdiz, Jose Luis
Vázquez Lasa, Blanca
San Román, Julio
Gálvez Martín, Patricia
author_role author
author2 López Ruiz, Elena
López Donaire, María Luisa
Jiménez, Gema
Rosa Aguilar, María
Marchal, Juan Antonio
Perdiz, Jose Luis
Vázquez Lasa, Blanca
San Román, Julio
Gálvez Martín, Patricia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv interpenetrated polymer network
semi-IPN
methacrylated hyaluronic acid
chitosan
glycerylphytate
mesenchymal stem cell
topic interpenetrated polymer network
semi-IPN
methacrylated hyaluronic acid
chitosan
glycerylphytate
mesenchymal stem cell
description In the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant di erences between them regarding composition, morphology, and mechanical properties after physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN membranes. The biological performance was evaluated on primary human mesenchymal stem cells (hMSCs) and the systems revealed no cytotoxic e ect. The excellent biocompatibility of the systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces. Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN, which suggested that these polymeric membranes could be proposed as an e ective promoter system of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes can provide a stable and amenable environment for hMSCs support and growth with potential applications in the biomedical field.
publishDate 2020
dc.date.none.fl_str_mv 2020
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10953/2350
url https://hdl.handle.net/10953/2350
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Polymers
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
instname:Universidad de Jaén
instname_str Universidad de Jaén
reponame_str RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
collection RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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