Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering
n the present study, semi- and interpenetrated polymer network (IPN) systems based onhyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker,glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized andevaluated as potential...
| Autores: | , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/231631 |
| Acceso en línea: | http://hdl.handle.net/10261/231631 |
| Access Level: | acceso abierto |
| Palabra clave: | interpenetrated polymer network semi-IPN methacrylated hyaluronic acid Chitosan glycerylphytate mesenchymal stem cel |
| Sumario: | n the present study, semi- and interpenetrated polymer network (IPN) systems based onhyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker,glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized andevaluated as potential supports for tissue engineering. Semi- and IPN systems showed significantdifferences between them regarding composition, morphology, and mechanical properties afterphysicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retentionand mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPNmembranes. The biological performance was evaluated on primary human mesenchymal stemcells (hMSCs) and the systems revealed no cytotoxic effect. The excellent biocompatibility of thesystems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces.Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN,which suggested that these polymeric membranes could be proposed as an effective promoter systemof tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranescan provide a stable and amenable environment for hMSCs support and growth with potentialapplications in the biomedical field. |
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