Betanodavirus genotypes produce clinical signs and mortality in the shi drum (Umbrina cirrosa), and infective particles are isolated from the damaged brain
Betanodavirus (NNV), the causative agent of viral retinopathy and encephalopathy (VER) disease in fish, is the greatest viral threat for aquaculture growth and diversification in the Mediterranean area, where several genotypes and reassortants have been described. The shi drum (Umbrina cirrosa) is a...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/313901 |
| Acceso en línea: | http://hdl.handle.net/10261/313901 |
| Access Level: | acceso abierto |
| Palabra clave: | Betanodavirus Centro Oceanográfico de Murcia Acuicultura Mediterranean fish Viral diseases Clinical signs Viral load Shi drum Mortalities fish genotypes cytology histology brain |
| Sumario: | Betanodavirus (NNV), the causative agent of viral retinopathy and encephalopathy (VER) disease in fish, is the greatest viral threat for aquaculture growth and diversification in the Mediterranean area, where several genotypes and reassortants have been described. The shi drum (Umbrina cirrosa) is an excellent candidate for marine aquaculture diversification in the Mediterranean area, as this species is easily adapted to culture conditions and shows high growth rates, low mortalities during larval development and high market value. Although outbreaks of RGNNV, the most common NNV genotype in the Mediterranean Sea, have been detected in wild and farmed shi drums, little is known about host–virus interactions, clinical signs, mortality rates and viral load in this species. In this framework, we have evaluated the mortality rates, the signs of the VER disease including behaviour, histopathological alterations in the brain and retina and the rescue of infective particles in shi drums after infection with the four NNV genotypes (RGNNV, SJNNV, BFNNV and TPNNV). We found that all of the genotypes produce mortalities and analogous time-lapses between the first signs of disease and mortalities. However, infective particles were only recovered from RGNNV-, BFNNV- and TPNNV-infected specimens. Interestingly, clinical signs and histopathological lesions in the brain and retina were different depending on the genotype used. |
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