A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.

Crego Navazo, Antonio Luis|||0000-0002-4083-5294; Sánchez Hernández, Laura; Marina Alegre, María Luisa|||0000-0002-5583-1624

A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.

Accepted, revised and published in "Journal of Cromatography A", 2001, 1218 (30), pp. 4944-4951. DOI: 10.1016/j.chroma.2011.01.045

Bibliographic Details
Authors:	Crego Navazo, Antonio Luis\|\|\|0000-0002-4083-5294, Sánchez Hernández, Laura, Marina Alegre, María Luisa\|\|\|0000-0002-5583-1624
Format:	article
Publication Date:	2011
Country:	España
Institution:	Universidad de Alcalá (UAH)
Repository:	e_Buah Biblioteca Digital Universidad de Alcalá
Language:	English
OAI Identifier:	oai:ebuah.uah.es:10017/21124
Online Access:	http://hdl.handle.net/10017/21124 https://dx.doi.org/10.1016/j.chroma.2011.01.045
Access Level:	Open access
Keyword:	Capillary electrophoresis Corn Mass spectrometry Non-protein amino acid Oil Olive Soybean Sunflower Ciencia Química Science Chemistry

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network_acronym_str	ES
network_name_str	España
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spelling	A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.Crego Navazo, Antonio Luis\|\|\|0000-0002-4083-5294Sánchez Hernández, LauraMarina Alegre, María Luisa\|\|\|0000-0002-5583-1624Capillary electrophoresisCornMass spectrometryNon-protein amino acidOilOliveSoybeanSunflowerCienciaQuímicaScienceChemistryAccepted, revised and published in "Journal of Cromatography A", 2001, 1218 (30), pp. 4944-4951. DOI: 10.1016/j.chroma.2011.01.045A new analytical methodology based on capillary electrophoresis–mass spectrometry (CE–MS2) is presented in this work, enabling the identification and determination of six non-protein amino acids (ornithine, β-alanine, GABA, alloisoleucine, citrulline and pyroglutamic acid) in vegetable oils. This methodology is based on a previous derivatization with butanol and subsequent separation using acidic conditions followed by on-line coupling to an ion trap analyzer for MS2 detection established through an electrospray-coaxial sheath flow interface. The electrophoretic and interface parameters were optimized obtaining the separation of all compounds in less than 15 min and with resolutions higher than 5. The proposed method was validated by assessing its accuracy, precision (RSD < 7% for corrected peak areas), LODs and LOQs (between 0.04–0.19 ng/g and 0.06–0.31 ng/g, respectively) and linearity range (R2 > 0.99), and it was used in order to identify the selected non-protein amino acids in soybean oils, sunflower oils, corn oils and extra virgin olive oils. MS2 experiments performed the fingerprint fragmentation of these compounds allowing to corroborate ornithine and alloisoleucine in seed oils but not in olive oils. The method was applied to identify and quantify olive oil adulterations with soybean oil detecting in a single run the amino acids in mixtures up to 2% (w/w). The results showed a high potential in using these compounds as novel markers for the detection of adulterations of extra virgin olive oils with seed oils. Thus, the developed method could be considered a simple, rapid and reliable method for the quality evaluation of extra virgin olive oil permitting its authentication.Acknowledgements Authors thank the Spanish Ministry of Science and Innovation (project CTQ2009-09022) and the Comunidad Autónoma of Madrid (Spain) and European funding from FEDER programme (project S2009/AGR-1464, ANALISYC-II). They also thank the University of Alcalá and the Comunidad Autónoma of Madrid for project CCG10-UAH/AGR-5950. Laura Sánchez-Hernández thanks the Comunidad Autónoma of Madrid for her research contractComunidad de MadridMinisterio de Ciencia e InnovaciónElsevier20112011-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/21124https://dx.doi.org/10.1016/j.chroma.2011.01.045reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available CCG10-UAH ESMICIN Not available CTQ2009-09022 ESComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available S2009 ESopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/211242026-06-18T11:13:07Z
dc.title.none.fl_str_mv	A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.
title	A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.
spellingShingle	A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils. Crego Navazo, Antonio Luis\|\|\|0000-0002-4083-5294 Capillary electrophoresis Corn Mass spectrometry Non-protein amino acid Oil Olive Soybean Sunflower Ciencia Química Science Chemistry
title_short	A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.
title_full	A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.
title_fullStr	A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.
title_full_unstemmed	A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.
title_sort	A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.
dc.creator.none.fl_str_mv	Crego Navazo, Antonio Luis\|\|\|0000-0002-4083-5294 Sánchez Hernández, Laura Marina Alegre, María Luisa\|\|\|0000-0002-5583-1624
author	Crego Navazo, Antonio Luis\|\|\|0000-0002-4083-5294
author_facet	Crego Navazo, Antonio Luis\|\|\|0000-0002-4083-5294 Sánchez Hernández, Laura Marina Alegre, María Luisa\|\|\|0000-0002-5583-1624
author_role	author
author2	Sánchez Hernández, Laura Marina Alegre, María Luisa\|\|\|0000-0002-5583-1624
author2_role	author author
dc.subject.none.fl_str_mv	Capillary electrophoresis Corn Mass spectrometry Non-protein amino acid Oil Olive Soybean Sunflower Ciencia Química Science Chemistry
topic	Capillary electrophoresis Corn Mass spectrometry Non-protein amino acid Oil Olive Soybean Sunflower Ciencia Química Science Chemistry
description	Accepted, revised and published in "Journal of Cromatography A", 2001, 1218 (30), pp. 4944-4951. DOI: 10.1016/j.chroma.2011.01.045
publishDate	2011
dc.date.none.fl_str_mv	2011 2011-01-01
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501 NA http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	http://hdl.handle.net/10017/21124 https://dx.doi.org/10.1016/j.chroma.2011.01.045
url	http://hdl.handle.net/10017/21124 https://dx.doi.org/10.1016/j.chroma.2011.01.045
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available CCG10-UAH ES MICIN Not available CTQ2009-09022 ES Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available S2009 ES
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	reponame:e_Buah Biblioteca Digital Universidad de Alcalá instname:Universidad de Alcalá (UAH)
instname_str	Universidad de Alcalá (UAH)
reponame_str	e_Buah Biblioteca Digital Universidad de Alcalá
collection	e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,300719

A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils.

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