Arbovirus Persistence In Mosquitoes: The Role Of Translation Control

Mosquito-transmitted viruses infect both vertebrate hosts, where they cause acute disease, and mosquito vectors, where they establish persistent infection. While persistence in mosquito cells is essential for transmission, the mechanisms enabling sustained viral protein expression without compromisi...

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Detalles Bibliográficos
Autor: Puig Torrents, Mireia
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/695463
Acceso en línea:http://hdl.handle.net/10803/695463
Access Level:acceso embargado
Palabra clave:Mosquito-transmitted virus
Persistence
Translation control
TRNA epitranscriptome
Infection stress response
578
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spelling Arbovirus Persistence In Mosquitoes: The Role Of Translation ControlPuig Torrents, MireiaMosquito-transmitted virusPersistenceTranslation controlTRNA epitranscriptomeInfection stress response578Mosquito-transmitted viruses infect both vertebrate hosts, where they cause acute disease, and mosquito vectors, where they establish persistent infection. While persistence in mosquito cells is essential for transmission, the mechanisms enabling sustained viral protein expression without compromising mosquito fitness remains poorly understood. Using chikungunya virus (CHIKV) as a model, this thesis addresses this fundamental question through a temporal, genome-wide analysis of transcription, translation, and tRNA modifications in both mosquito cell culture and in vivo. We find that persistence is characterized by a dynamic host-virus translational balance, wherein viral RNA translation is selectively repressed, allowing sufficient viral protein expression for transmission while preserving host protein synthesis and fitness. Crucially, this repression involves a tRNA modification–dependent mechanism that modulates translation in response to virus-induced metabolic stress. These findings reveal a novel strategy by which mosquito-transmitted viruses fine-tune the translational landscape of their vector to support long-term persistence and successful transmission.Universitat Pompeu Fabra. Doctorat en BiomedicinaUniversitat Pompeu FabraDíez Antón, JuanaTalló Parra, MarcDíez Antón, Juana2025202520252027info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion181 p.application/pdfhttp://hdl.handle.net/10803/695463TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésL'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-nd/4.0/http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/embargoedAccessoai:www.tdx.cat:10803/6954632026-06-14T12:46:07Z
dc.title.none.fl_str_mv Arbovirus Persistence In Mosquitoes: The Role Of Translation Control
title Arbovirus Persistence In Mosquitoes: The Role Of Translation Control
spellingShingle Arbovirus Persistence In Mosquitoes: The Role Of Translation Control
Puig Torrents, Mireia
Mosquito-transmitted virus
Persistence
Translation control
TRNA epitranscriptome
Infection stress response
578
title_short Arbovirus Persistence In Mosquitoes: The Role Of Translation Control
title_full Arbovirus Persistence In Mosquitoes: The Role Of Translation Control
title_fullStr Arbovirus Persistence In Mosquitoes: The Role Of Translation Control
title_full_unstemmed Arbovirus Persistence In Mosquitoes: The Role Of Translation Control
title_sort Arbovirus Persistence In Mosquitoes: The Role Of Translation Control
dc.creator.none.fl_str_mv Puig Torrents, Mireia
author Puig Torrents, Mireia
author_facet Puig Torrents, Mireia
author_role author
dc.contributor.none.fl_str_mv Díez Antón, Juana
Talló Parra, Marc
Díez Antón, Juana
dc.subject.none.fl_str_mv Mosquito-transmitted virus
Persistence
Translation control
TRNA epitranscriptome
Infection stress response
578
topic Mosquito-transmitted virus
Persistence
Translation control
TRNA epitranscriptome
Infection stress response
578
description Mosquito-transmitted viruses infect both vertebrate hosts, where they cause acute disease, and mosquito vectors, where they establish persistent infection. While persistence in mosquito cells is essential for transmission, the mechanisms enabling sustained viral protein expression without compromising mosquito fitness remains poorly understood. Using chikungunya virus (CHIKV) as a model, this thesis addresses this fundamental question through a temporal, genome-wide analysis of transcription, translation, and tRNA modifications in both mosquito cell culture and in vivo. We find that persistence is characterized by a dynamic host-virus translational balance, wherein viral RNA translation is selectively repressed, allowing sufficient viral protein expression for transmission while preserving host protein synthesis and fitness. Crucially, this repression involves a tRNA modification–dependent mechanism that modulates translation in response to virus-induced metabolic stress. These findings reveal a novel strategy by which mosquito-transmitted viruses fine-tune the translational landscape of their vector to support long-term persistence and successful transmission.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2027
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10803/695463
url http://hdl.handle.net/10803/695463
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv 181 p.
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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