Synthetic biology for microbiome-based genetic domestication of the skin and the sea

Microorganisms exhibit remarkable adaptability and represent a vast genetic resource. Synthetic biology aims to leverage this diversity for sustainability and medicine. However, many microbes remain unexplored due to limited genetic tools. This research developed genetic engineering tools for Dinoro...

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Author: Nevot Sánchez, Guillermo
Format: doctoral thesis
Status:Published version
Publication Date:2024
Country:España
Institution:CBUC, CESCA
Repository:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/692372
Online Access:http://hdl.handle.net/10803/692372
Access Level:Embargoed access
Keyword:Cutibacterium acnes
Dinoroseobacter shibae
Microbiome engineering
Synthetic biology
Skin
Biología sintética
Terapias bacterianas
Microbioma
Piel
Ingeniería genética
575
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spelling Synthetic biology for microbiome-based genetic domestication of the skin and the seaNevot Sánchez, GuillermoCutibacterium acnesDinoroseobacter shibaeMicrobiome engineeringSynthetic biologySkinBiología sintéticaTerapias bacterianasMicrobiomaPielIngeniería genética575Microorganisms exhibit remarkable adaptability and represent a vast genetic resource. Synthetic biology aims to leverage this diversity for sustainability and medicine. However, many microbes remain unexplored due to limited genetic tools. This research developed genetic engineering tools for Dinoroseobacter shibae and Cutibacterium acnes, abundant in the ocean and on the skin, respectively. For C. acnes, we investigated phage defenses and developed a genetic toolkit including expression tools, CRISPRi, biosensors, recombinases, and auxotrophic genes, facilitating its use in skin engineering. We created auxotrophic strains and an antioxidant strain reducing oxidative stress in UV-treated keratinocytes. For D. shibae, we engineered fimbrils to enhance biofilm adhesion and designed biosensors for temperature and oxygen, demonstrating its potential for marine applications. Additionally, we found that using preprints and inquiry-based lessons in teaching synthetic biology improved students' technical and soft skills, proving effective for interdisciplinary educationLos microorganismos poseen una gran adaptabilidad y representan un amplio recurso genético. La biología sintética busca aprovechar esta diversidad para la sostenibilidad y la medicina. Sin embargo, muchos microbios siguen sin explorarse debido la falta de herramientas genéticas. En este estudio, desarrollamos herramientas de ingeniería genética para Dinoroseobacter shibae y Cutibacterium acnes, abundantes en el océano y en la piel, respectivamente. Para C. acnes, investigamos las defensas contra fagos y desarrollamos un kit de herramientas genéticas que incluye herramientas de expresión, CRISPRi, biosensores, recombinasas y genes auxótrofos, facilitando su uso en la ingeniería del microbioma de la piel. Creamos cepas auxótrofas y una cepa antioxidante que reduce el estrés oxidativo en queratinocitos tratados con luz UV. Para D. shibae, diseñamos fimbrias para mejorar la adhesión de los biofilms y biosensores para temperatura y oxígeno, demostrando su potencial para aplicaciones marinas. Además, encontramos que el uso de preprints y lecciones basadas en la investigación mejoraron las habilidades técnicas y blandas de los estudiantes, demostrando ser efectivo para la educación interdisciplinarPrograma de Doctorat en BiomedicinaUniversitat Pompeu FabraGüell Cargol, MarcUniversitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida202420242026info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion211 p.application/pdfhttp://hdl.handle.net/10803/692372TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. Tots els drets reservats. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/embargoedAccessoai:www.tdx.cat:10803/6923722026-06-14T12:46:07Z
dc.title.none.fl_str_mv Synthetic biology for microbiome-based genetic domestication of the skin and the sea
title Synthetic biology for microbiome-based genetic domestication of the skin and the sea
spellingShingle Synthetic biology for microbiome-based genetic domestication of the skin and the sea
Nevot Sánchez, Guillermo
Cutibacterium acnes
Dinoroseobacter shibae
Microbiome engineering
Synthetic biology
Skin
Biología sintética
Terapias bacterianas
Microbioma
Piel
Ingeniería genética
575
title_short Synthetic biology for microbiome-based genetic domestication of the skin and the sea
title_full Synthetic biology for microbiome-based genetic domestication of the skin and the sea
title_fullStr Synthetic biology for microbiome-based genetic domestication of the skin and the sea
title_full_unstemmed Synthetic biology for microbiome-based genetic domestication of the skin and the sea
title_sort Synthetic biology for microbiome-based genetic domestication of the skin and the sea
dc.creator.none.fl_str_mv Nevot Sánchez, Guillermo
author Nevot Sánchez, Guillermo
author_facet Nevot Sánchez, Guillermo
author_role author
dc.contributor.none.fl_str_mv Güell Cargol, Marc
Universitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida
dc.subject.none.fl_str_mv Cutibacterium acnes
Dinoroseobacter shibae
Microbiome engineering
Synthetic biology
Skin
Biología sintética
Terapias bacterianas
Microbioma
Piel
Ingeniería genética
575
topic Cutibacterium acnes
Dinoroseobacter shibae
Microbiome engineering
Synthetic biology
Skin
Biología sintética
Terapias bacterianas
Microbioma
Piel
Ingeniería genética
575
description Microorganisms exhibit remarkable adaptability and represent a vast genetic resource. Synthetic biology aims to leverage this diversity for sustainability and medicine. However, many microbes remain unexplored due to limited genetic tools. This research developed genetic engineering tools for Dinoroseobacter shibae and Cutibacterium acnes, abundant in the ocean and on the skin, respectively. For C. acnes, we investigated phage defenses and developed a genetic toolkit including expression tools, CRISPRi, biosensors, recombinases, and auxotrophic genes, facilitating its use in skin engineering. We created auxotrophic strains and an antioxidant strain reducing oxidative stress in UV-treated keratinocytes. For D. shibae, we engineered fimbrils to enhance biofilm adhesion and designed biosensors for temperature and oxygen, demonstrating its potential for marine applications. Additionally, we found that using preprints and inquiry-based lessons in teaching synthetic biology improved students' technical and soft skills, proving effective for interdisciplinary education
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10803/692372
url http://hdl.handle.net/10803/692372
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv 211 p.
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405769003171840
score 15,811543

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