Synthetic biology for microbiome-based genetic domestication of the skin and the sea
Microorganisms exhibit remarkable adaptability and represent a vast genetic resource. Synthetic biology aims to leverage this diversity for sustainability and medicine. However, many microbes remain unexplored due to limited genetic tools. This research developed genetic engineering tools for Dinoro...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/692372 |
| Acceso en línea: | http://hdl.handle.net/10803/692372 |
| Access Level: | acceso embargado |
| Palabra clave: | Cutibacterium acnes Dinoroseobacter shibae Microbiome engineering Synthetic biology Skin Biología sintética Terapias bacterianas Microbioma Piel Ingeniería genética 575 |
| Sumario: | Microorganisms exhibit remarkable adaptability and represent a vast genetic resource. Synthetic biology aims to leverage this diversity for sustainability and medicine. However, many microbes remain unexplored due to limited genetic tools. This research developed genetic engineering tools for Dinoroseobacter shibae and Cutibacterium acnes, abundant in the ocean and on the skin, respectively. For C. acnes, we investigated phage defenses and developed a genetic toolkit including expression tools, CRISPRi, biosensors, recombinases, and auxotrophic genes, facilitating its use in skin engineering. We created auxotrophic strains and an antioxidant strain reducing oxidative stress in UV-treated keratinocytes. For D. shibae, we engineered fimbrils to enhance biofilm adhesion and designed biosensors for temperature and oxygen, demonstrating its potential for marine applications. Additionally, we found that using preprints and inquiry-based lessons in teaching synthetic biology improved students' technical and soft skills, proving effective for interdisciplinary education |
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