Synthetically programmed antioxidant delivery by a domesticated skin commensal
Bacteria represent a promising dynamic delivery system for the treatment of disease. In the skin, the relevant location of Cutibacterium acnes within the hair follicle makes this bacterium an attractive chassis for dermal biotechnological applications. Here, we provide a genetic toolbox for the engi...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universitat Pompeu Fabra |
| Repositorio: | Repositorio Digital de la UPF |
| OAI Identifier: | oai:repositori.upf.edu:10230/69961 |
| Acceso en línea: | http://hdl.handle.net/10230/69961 http://dx.doi.org/10.1016/j.cels.2025.101169 |
| Access Level: | acceso abierto |
| Palabra clave: | CRISPRi Cutibacterium acnes SOD Auxotrophies Genetic toolkit Keratinocytes Microbiome engineering Plasmid Skin Synthetic biology |
| Sumario: | Bacteria represent a promising dynamic delivery system for the treatment of disease. In the skin, the relevant location of Cutibacterium acnes within the hair follicle makes this bacterium an attractive chassis for dermal biotechnological applications. Here, we provide a genetic toolbox for the engineering of this traditionally intractable bacterium, including basic gene expression tools, biocontainment strategies, markerless genetic engineering, and dynamic transcriptional regulation. As a proof of concept, we develop an antioxidant-secreting strain capable of reducing oxidative stress in a UV stress model. |
|---|