Glutathione redox state plays a key role in flower development and pollen vigour

The importance of the glutathione pool in the development of reproductive tissues and in pollen tube growth was investigated in wild-type (WT) Arabidopsis thaliana, a reporter line expressing redox-sensitive green fluorescent protein (roGFP2), and a glutathione-deficient cad2-1 mutant (cad2-1/roGFP2...

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Autores: García-Quirós, Estefanía, Alché Ramírez, Juan de Dios, Karpinska, B., Foyer, Christine H., Wilson, Z.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/216254
Acceso en línea:http://hdl.handle.net/10261/216254
Access Level:acceso abierto
Palabra clave:Flower development
Glutathione
Oxidative stress
Pollen germination
Redox regulation
Redox-sensitive green fluorescent protein (roGFP)
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spelling Glutathione redox state plays a key role in flower development and pollen vigourGarcía-Quirós, EstefaníaAlché Ramírez, Juan de DiosKarpinska, B.Foyer, Christine H.Wilson, Z.Flower developmentGlutathioneOxidative stressPollen germinationRedox regulationRedox-sensitive green fluorescent protein (roGFP)The importance of the glutathione pool in the development of reproductive tissues and in pollen tube growth was investigated in wild-type (WT) Arabidopsis thaliana, a reporter line expressing redox-sensitive green fluorescent protein (roGFP2), and a glutathione-deficient cad2-1 mutant (cad2-1/roGFP2). The cad2-1/roGFP2 flowers had significantly less reduced glutathione (GSH) and more glutathione disulfide (GSSG) than WT or roGFP2 flowers. The stigma, style, anther, germinated pollen grains, and pollen tubes of roGFP2 flowers had a low degree of oxidation. However, these tissues were more oxidized in cad2-1/roGFP2 flowers than the roGFP2 controls. The ungerminated pollen grains were significantly more oxidized than the germinated pollen grains, indicating that the pollen cells become reduced upon the transition from the quiescent to the metabolically active state during germination. The germination percentage was lower in cad2-1/roGFP2 pollen and pollen tube growth arrested earlier than in roGFP2 pollen, demonstrating that increased cellular reduction is essential for pollen tube growth. These findings establish that ungerminated pollen grains exist in a relatively oxidized state compared with germinating pollen grains. Moreover, failure to accumulate glutathione and maintain a high GSH/GSSG ratio has a strong negative effect on pollen germination.This work was supported by European Regional Development Fund-co-funded projects BFU2016-77243-P, P2011-CVI7487, RTC2016-4824-2, and RTC2017-6654-2. EGQ thanks the Spanish Ministry of Economy and Competitiveness for “Formación del Personal Investigador” programgrant fundingOxford University PressMinisterio de Economía y Competitividad (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/216254reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC-2017-6654-2info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-77243-Pinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC-2016-4824-2http://dx.doi.org/10.1093/jxb/erz376Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2162542026-05-22T06:33:51Z
dc.title.none.fl_str_mv Glutathione redox state plays a key role in flower development and pollen vigour
title Glutathione redox state plays a key role in flower development and pollen vigour
spellingShingle Glutathione redox state plays a key role in flower development and pollen vigour
García-Quirós, Estefanía
Flower development
Glutathione
Oxidative stress
Pollen germination
Redox regulation
Redox-sensitive green fluorescent protein (roGFP)
title_short Glutathione redox state plays a key role in flower development and pollen vigour
title_full Glutathione redox state plays a key role in flower development and pollen vigour
title_fullStr Glutathione redox state plays a key role in flower development and pollen vigour
title_full_unstemmed Glutathione redox state plays a key role in flower development and pollen vigour
title_sort Glutathione redox state plays a key role in flower development and pollen vigour
dc.creator.none.fl_str_mv García-Quirós, Estefanía
Alché Ramírez, Juan de Dios
Karpinska, B.
Foyer, Christine H.
Wilson, Z.
author García-Quirós, Estefanía
author_facet García-Quirós, Estefanía
Alché Ramírez, Juan de Dios
Karpinska, B.
Foyer, Christine H.
Wilson, Z.
author_role author
author2 Alché Ramírez, Juan de Dios
Karpinska, B.
Foyer, Christine H.
Wilson, Z.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Flower development
Glutathione
Oxidative stress
Pollen germination
Redox regulation
Redox-sensitive green fluorescent protein (roGFP)
topic Flower development
Glutathione
Oxidative stress
Pollen germination
Redox regulation
Redox-sensitive green fluorescent protein (roGFP)
description The importance of the glutathione pool in the development of reproductive tissues and in pollen tube growth was investigated in wild-type (WT) Arabidopsis thaliana, a reporter line expressing redox-sensitive green fluorescent protein (roGFP2), and a glutathione-deficient cad2-1 mutant (cad2-1/roGFP2). The cad2-1/roGFP2 flowers had significantly less reduced glutathione (GSH) and more glutathione disulfide (GSSG) than WT or roGFP2 flowers. The stigma, style, anther, germinated pollen grains, and pollen tubes of roGFP2 flowers had a low degree of oxidation. However, these tissues were more oxidized in cad2-1/roGFP2 flowers than the roGFP2 controls. The ungerminated pollen grains were significantly more oxidized than the germinated pollen grains, indicating that the pollen cells become reduced upon the transition from the quiescent to the metabolically active state during germination. The germination percentage was lower in cad2-1/roGFP2 pollen and pollen tube growth arrested earlier than in roGFP2 pollen, demonstrating that increased cellular reduction is essential for pollen tube growth. These findings establish that ungerminated pollen grains exist in a relatively oxidized state compared with germinating pollen grains. Moreover, failure to accumulate glutathione and maintain a high GSH/GSSG ratio has a strong negative effect on pollen germination.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/216254
url http://hdl.handle.net/10261/216254
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC-2017-6654-2
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-77243-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC-2016-4824-2
http://dx.doi.org/10.1093/jxb/erz376
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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