Reversible cysteine oxidation in hydrogen peroxide sensing and signal transduction

Activation of redox cascades through hydrogen peroxide-mediated reversible cysteine oxidation is a major mechanism for intracellular signaling. Understanding why some cysteine residues are specifically oxidized, in competition with other proximal cysteine residues and in the presence of strong redox...

ver descrição completa

Detalhes bibliográficos
Autores: García Santamarina, Sarela, 1978-, Boronat i Llop, Susanna, 1965-, Hidalgo Hernando, Elena
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/25566
Acesso em linha:http://hdl.handle.net/10230/25566
http://dx.doi.org/10.1021/bi401700f
Access Level:acceso abierto
Palavra-chave:Oxigen actiu
Proteòmica
Redox biology
H2O2 reactivity
Cys oxidation
H2O2 sensor
Redox proteomics
Pap1
Yap1
OxyR
Nrf2
roGFP
Descrição
Resumo:Activation of redox cascades through hydrogen peroxide-mediated reversible cysteine oxidation is a major mechanism for intracellular signaling. Understanding why some cysteine residues are specifically oxidized, in competition with other proximal cysteine residues and in the presence of strong redox buffers, is therefore crucial for understanding redox signaling. In this review, we explore the recent advances in thiol-redox chemistry linked to signaling. We describe the last findings in the field of redox sensors, those that are naturally present in different model organisms as well as those that have been engineered to quantify intracellular hydrogen peroxide concentrations. Finally, we provide a summary of the newest approaches developed to study reversible cysteine oxidation at the proteomic level.