Proteomics analysis in frozen horse mackerel previously high-pressure processed

8 páginas, 4 figuras, 2 tablas

Bibliographic Details
Authors: Pazos Palmeiro, Manuel, Méndez, Lucía, Vázquez, Manuel, Aubourg, Santiago P.
Format: article
Status:Versión aceptada para publicación
Publication Date:2015
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/124682
Online Access:http://hdl.handle.net/10261/124682
Access Level:Open access
Keyword:Trachurus trachurus
High pressure
Frozen storage
SDS-soluble proteins
Sarcoplasmic proteins
Proteomics
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spelling Proteomics analysis in frozen horse mackerel previously high-pressure processedPazos Palmeiro, ManuelMéndez, LucíaVázquez, ManuelAubourg, Santiago P.Trachurus trachurusHigh pressureFrozen storageSDS-soluble proteinsSarcoplasmic proteinsProteomics8 páginas, 4 figuras, 2 tablasThe effect of high-pressure processing (HPP) (150, 300 and 450 MPa for 0, 2.5 and 5 min) on total sodium dodecyl sulphate (SDS)-soluble and sarcoplasmic proteins in frozen (−10 °C for 3 months) horse mackerel (Trachurus trachurus) was evaluated. Proteomics tools based on image analysis of SDS–PAGE protein gels and protein identification by tandem mass spectrometry (MS/MS) were applied. Although total SDS-soluble fraction indicated no important changes induced by HPP, this processing modified the 1-D SDS–PAGE sarcoplasmic patterns in a direct-dependent manner and exerted a selective effect on particular proteins depending on processing conditions. Thus, application of the highest pressure (450 MPa) provoked a significant degradation of phosphoglycerate mutase 2, glycogen phosphorylase muscle form, pyruvate kinase muscle isozyme, beta-enolase and triosephosphate isomerase and phosphoglucomutase-1. Conversely, protein bands assigned to tropomyosin alpha-1 chain, fast myotomal muscle troponin T and parvalbumin beta 2 increased their intensity after applying a 450-MPa processingThe Xunta de Galicia (Galicia, Spain) and the European Social Fund (Belgium) are thankfully recognised by the financial support of the postdoctoral ‘‘Isidro Parga Pondal’’ contract to M.P. The Spanish Ministry of Science and Innovation (Spain) is also grate- fully acknowledged for the doctoral fellowship to L.M. This work was supported by the Secretaría Xeral de I+D from the Xunta de Galicia (Galicia, Spain) through the Research Project 10TAL402001PR (2010–2012).Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/124682reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.foodchem.2015.03.144Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1246822026-05-22T06:33:51Z
dc.title.none.fl_str_mv Proteomics analysis in frozen horse mackerel previously high-pressure processed
title Proteomics analysis in frozen horse mackerel previously high-pressure processed
spellingShingle Proteomics analysis in frozen horse mackerel previously high-pressure processed
Pazos Palmeiro, Manuel
Trachurus trachurus
High pressure
Frozen storage
SDS-soluble proteins
Sarcoplasmic proteins
Proteomics
title_short Proteomics analysis in frozen horse mackerel previously high-pressure processed
title_full Proteomics analysis in frozen horse mackerel previously high-pressure processed
title_fullStr Proteomics analysis in frozen horse mackerel previously high-pressure processed
title_full_unstemmed Proteomics analysis in frozen horse mackerel previously high-pressure processed
title_sort Proteomics analysis in frozen horse mackerel previously high-pressure processed
dc.creator.none.fl_str_mv Pazos Palmeiro, Manuel
Méndez, Lucía
Vázquez, Manuel
Aubourg, Santiago P.
author Pazos Palmeiro, Manuel
author_facet Pazos Palmeiro, Manuel
Méndez, Lucía
Vázquez, Manuel
Aubourg, Santiago P.
author_role author
author2 Méndez, Lucía
Vázquez, Manuel
Aubourg, Santiago P.
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Trachurus trachurus
High pressure
Frozen storage
SDS-soluble proteins
Sarcoplasmic proteins
Proteomics
topic Trachurus trachurus
High pressure
Frozen storage
SDS-soluble proteins
Sarcoplasmic proteins
Proteomics
description 8 páginas, 4 figuras, 2 tablas
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/124682
url http://hdl.handle.net/10261/124682
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.foodchem.2015.03.144
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405258520723456
score 15,811543

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