Identification of proteins in excretory/secretory extracts of Echinostoma friedi (Trematoda) from chronic and acute infections

In the present study, we describe the investigation of Echinostoma friedi excretory/secretory products using a proteomic approach combined with the use of heterologous antibodies. We have identified 18 protein spots corresponding to ten proteins, including cytoskeletal proteins like actin, tropomyos...

Descripción completa

Detalles Bibliográficos
Autores: Bernal, Dolores, Carpena, Inés, Espert, Ana María, de la Rubia Ortí, José Enrique, Esteban, José Guillermo, Toledo, Rafael, Marcilla, Antonio
Tipo de recurso: artículo
Fecha de publicación:2006
País:España
Institución:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/4483
Acceso en línea:http://hdl.handle.net/20.500.12466/4483
Access Level:acceso abierto
Palabra clave:Echinostoma friedi
Excretory
Secretory extracts
32 Ciencias Médicas
Descripción
Sumario:In the present study, we describe the investigation of Echinostoma friedi excretory/secretory products using a proteomic approach combined with the use of heterologous antibodies. We have identified 18 protein spots corresponding to ten proteins, including cytoskeletal proteins like actin, tropomyosin, and paramyosin; glycolytic enzymes like enolase, glyceraldehyde 3P dehydrogenase, and aldolase; detoxifying enzymes like GSTs; and stress proteins like heat shock protein (Hsp) 70. Among these proteins, both actin and, to a lesser extent, Hsp70, exhibited differential expression patterns between chronic and acute infections in the Echinostoma–rodent model, suggesting that these proteins may play a role in the survival within the host.