Identification of enolase as a plasminogen-binding protein in excretory-secretory products of Fasciola hepatica
We have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host^parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we have identi¢ed the enolase enzyme, present in the excret...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2004 |
| País: | España |
| Institución: | Universidad Católica de Valencia San Vicente Mártir |
| Repositorio: | RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir |
| Idioma: | inglés |
| OAI Identifier: | oai:riucv.ucv.es:20.500.12466/4481 |
| Acceso en línea: | http://hdl.handle.net/20.500.12466/4481 |
| Access Level: | acceso abierto |
| Palabra clave: | Excretory Secretory Enolase Plasminogen binding Fasciola hepatica 32 Ciencias Médicas |
| Sumario: | We have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host^parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we have identi¢ed the enolase enzyme, present in the excretory/ secretory materials of F. hepatica, as a human plasminogen- binding protein. This enzyme has an apparent molecular weight of 47 kDa with pI ranging from 6.2 to 7.2. These results suggest that enolase could act as a plasminogen receptor. |
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