Cross-sectional evaluation of circulating hepatitis B virus RNA and DNA

Different forms of pregenomic and other hepatitis B virus (HBV) RNA have been detected in patients' sera. These circulating HBV-RNAs may be useful for monitoring covalently closed circular DNA activity, and predicting hepatitis B e-antigen seroconversion or viral rebound after nucleos(t)ide ana...

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Authors: García-García, Selene|||0000-0001-7697-2058, Cortese, Maria Francesca|||0000-0002-4318-532X, Tabernero, David|||0000-0002-1146-4084, Gregori i Font, Josep|||0000-0002-4253-8015, Vila, Marta|||0000-0001-9303-5189, Pacín Ruiz, Beatriz|||0000-0001-8718-6589, Quer, Josep|||0000-0003-0014-084X, Casillas, Rosario|||0000-0002-6758-6734, Castillo-Ribelles, Laura|||0000-0001-9380-7839, Ferrer Costa, Roser|||0000-0002-8925-3172, Rando-Segura, Ariadna|||0000-0003-4555-7286, Trejo-Zahínos, Jesús, Pumarola Suñé, Tomàs|||0000-0002-5171-7461, Casis-Saez, Ernesto, Esteban, Rafael|||0000-0001-5280-392X, Riveiro Barciela, Mar|||0000-0001-9309-2052, Buti, Maria|||0000-0002-0732-3078, Rodríguez Frías, Francisco|||0000-0002-9128-7013
Format: article
Publication Date:2021
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:282997
Online Access:https://ddd.uab.cat/record/282997
https://dx.doi.org/urn:doi:10.3748/wjg.v27.i41.7144
Access Level:Open access
Keyword:Hepatitis B virus RNA
Hepatitis B X gene
Quasispecies
Next-generation sequencing
Quasispecies conservation
Quasispecies complexity
Description
Summary:Different forms of pregenomic and other hepatitis B virus (HBV) RNA have been detected in patients' sera. These circulating HBV-RNAs may be useful for monitoring covalently closed circular DNA activity, and predicting hepatitis B e-antigen seroconversion or viral rebound after nucleos(t)ide analog cessation. Data on serum HBV-RNA quasispecies, however, is scarce. It is therefore important to develop methodologies to thoroughly analyze this quasispecies, ensuring the elimination of any residual HBV-DNA. Studying circulating HBV-RNA quasispecies may facilitate achieving functional cure of HBV infection. To establish a next-generation sequencing (NGS) methodology for analyzing serum HBV-RNA and comparing it with DNA quasispecies. Thirteen untreated chronic hepatitis B patients, showing different HBV-genotypes and degrees of severity of liver disease were enrolled in the study and a serum sample with HBV-DNA.

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