Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty rats

This article belongs to the Special Issue Effects of Phytochemicals on Metabolic Disorders and Human Health.

Detalles Bibliográficos
Autores: Gallardo-Villanueva, Paula, Fernández-Marcelo, Tamara, Villamayor, Laura, Valverde, Ángela M., Ramos, Sonia, Fernández-Millán, Elisa, Martín, M. Ángeles
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/358768
Acceso en línea:http://hdl.handle.net/10261/358768
Access Level:acceso abierto
Palabra clave:Type 2 diabetes
Flavonoids
Pancreatic beta cells
Inflammation
Macrophages
Oxidative stress
id ES_1e5e400ebb7b76366a1a39dfd1310559
oai_identifier_str oai:digital.csic.es:10261/358768
network_acronym_str ES
network_name_str España
repository_id_str
spelling Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty ratsGallardo-Villanueva, PaulaFernández-Marcelo, TamaraVillamayor, LauraValverde, Ángela M.Ramos, SoniaFernández-Millán, ElisaMartín, M. ÁngelesType 2 diabetesFlavonoidsPancreatic beta cellsInflammationMacrophagesOxidative stressThis article belongs to the Special Issue Effects of Phytochemicals on Metabolic Disorders and Human Health.The loss of functional beta-cell mass in diabetes is directly linked to the development of diabetic complications. Although dietary flavonoids have demonstrated antidiabetic properties, their potential effects on pancreatic beta-cell preservation and their synergistic benefits with antidiabetic drugs remain underexplored. We have developed a potential functional food enriched in flavonoids by combining cocoa powder and carob flour (CCB), which has shown antidiabetic effects. Here, we investigated the ability of the CCB, alone or in combination with metformin, to preserve pancreatic beta cells in an established diabetic context and their potential synergistic effect. Zucker diabetic fatty rats (ZDF) were fed a CCB-rich diet or a control diet, with or without metformin, for 12 weeks. Markers of pancreatic oxidative stress and inflammation, as well as relative beta-cell mass and beta-cell apoptosis, were analyzed. Results demonstrated that CCB feeding counteracted pancreatic oxidative stress by enhancing the antioxidant defense and reducing reactive oxygen species. Moreover, the CCB suppressed islet inflammation by preventing macrophage infiltration into islets and overproduction of pro-inflammatory cytokines, along with the inactivation of nuclear factor kappa B (NFκB). As a result, the CCB supplementation prevented beta-cell apoptosis and the loss of beta cells in ZDF diabetic animals. The observed additive effect when combining the CCB with metformin underscores its potential as an adjuvant therapy to delay the progression of type 2 diabetes.This work was supported by the grants RTI2018-095059-B-I00 and PID2020-116134RB-I00 (MCIN/AEI/10.13039/501100011033/ and by “ERDF A way of making Europe”), grant P2022/BMD-7227 (Comunidad de Madrid, Spain) and grant DEM2022/PIM01 by CIBERDEM (CB07/08/0013 and CB07/08/0033), Instituto de Salud Carlos III, MCIN.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)European CommissionMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Instituto de Salud Carlos IIIComunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/358768reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095059-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116134RB-I00P2022/BMD-7227/MOIR-ACTOME-CMThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/nu16020273https://doi.org/10.3390/nu16020273Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3587682026-05-22T06:33:51Z
dc.title.none.fl_str_mv Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty rats
title Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty rats
spellingShingle Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty rats
Gallardo-Villanueva, Paula
Type 2 diabetes
Flavonoids
Pancreatic beta cells
Inflammation
Macrophages
Oxidative stress
title_short Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty rats
title_full Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty rats
title_fullStr Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty rats
title_full_unstemmed Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty rats
title_sort Synergistic effect of a flavonoid-rich cocoa–carob blend and metformin in preserving pancreatic beta cells in Zucker diabetic fatty rats
dc.creator.none.fl_str_mv Gallardo-Villanueva, Paula
Fernández-Marcelo, Tamara
Villamayor, Laura
Valverde, Ángela M.
Ramos, Sonia
Fernández-Millán, Elisa
Martín, M. Ángeles
author Gallardo-Villanueva, Paula
author_facet Gallardo-Villanueva, Paula
Fernández-Marcelo, Tamara
Villamayor, Laura
Valverde, Ángela M.
Ramos, Sonia
Fernández-Millán, Elisa
Martín, M. Ángeles
author_role author
author2 Fernández-Marcelo, Tamara
Villamayor, Laura
Valverde, Ángela M.
Ramos, Sonia
Fernández-Millán, Elisa
Martín, M. Ángeles
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Instituto de Salud Carlos III
Comunidad de Madrid
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Type 2 diabetes
Flavonoids
Pancreatic beta cells
Inflammation
Macrophages
Oxidative stress
topic Type 2 diabetes
Flavonoids
Pancreatic beta cells
Inflammation
Macrophages
Oxidative stress
description This article belongs to the Special Issue Effects of Phytochemicals on Metabolic Disorders and Human Health.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/358768
url http://hdl.handle.net/10261/358768
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095059-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116134RB-I00
P2022/BMD-7227/MOIR-ACTOME-CM
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/nu16020273
https://doi.org/10.3390/nu16020273
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404332453003264
score 15.81155

Similares en LA Referencia