Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.

Meis1 and Meis2 are homeodomain transcription factors that regulate organogenesis through cooperation with Hox proteins. Elimination of Meis genes after limb induction has shown their role in limb proximo-distal patterning; however, limb development in the complete absence of Meis function has not b...

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Detalles Bibliográficos
Autores: Delgado, Irene, Giovinazzo, Giovanna, Temiño, Susana, Gauthier, Yves, Balsalobre, Aurelio, Drouin, Jacques, Torres, Miguel
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/15251
Acceso en línea:http://hdl.handle.net/20.500.12105/15251
Access Level:acceso abierto
Palabra clave:Animals
Basic Helix-Loop-Helix Transcription Factors
Body Patterning
DNA-Binding Proteins
Fibroblast Growth Factor 10
Hedgehog Proteins
Homeodomain Proteins
Limb Buds
Mice, Knockout
Mice, Transgenic
Models, Genetic
Myeloid Ecotropic Viral Integration Site 1 Protein
Neoplasm Proteins
T-Box Domain Proteins
Transcription Factors
Transcriptional Activation
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spelling Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.Delgado, IreneGiovinazzo, GiovannaTemiño, SusanaGauthier, YvesBalsalobre, AurelioDrouin, JacquesTorres, MiguelAnimalsBasic Helix-Loop-Helix Transcription FactorsBody PatterningDNA-Binding ProteinsFibroblast Growth Factor 10Hedgehog ProteinsHomeodomain ProteinsLimb BudsMice, KnockoutMice, TransgenicModels, GeneticMyeloid Ecotropic Viral Integration Site 1 ProteinNeoplasm ProteinsT-Box Domain ProteinsTranscription FactorsTranscriptional ActivationMeis1 and Meis2 are homeodomain transcription factors that regulate organogenesis through cooperation with Hox proteins. Elimination of Meis genes after limb induction has shown their role in limb proximo-distal patterning; however, limb development in the complete absence of Meis function has not been studied. Here, we report that Meis1/2 inactivation in the lateral plate mesoderm of mouse embryos leads to limb agenesis. Meis and Tbx factors converge in this function, extensively co-binding with Tbx to genomic sites and co-regulating enhancers of Fgf10, a critical factor in limb initiation. Limbs with three deleted Meis alleles show proximal-specific skeletal hypoplasia and agenesis of posterior skeletal elements. This failure in posterior specification results from an early role of Meis factors in establishing the limb antero-posterior prepattern required for Shh activation. Our results demonstrate roles for Meis transcription factors in early limb development and identify their involvement in previously undescribed interaction networks that regulate organogenesis.Nature Publishing GroupMinisterio de Ciencia e Innovación (España)Fundación ProCNIC20222022-12-0120212021-05-2520212021-05-25journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/15251reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/152512026-06-12T12:43:37Z
dc.title.none.fl_str_mv Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.
title Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.
spellingShingle Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.
Delgado, Irene
Animals
Basic Helix-Loop-Helix Transcription Factors
Body Patterning
DNA-Binding Proteins
Fibroblast Growth Factor 10
Hedgehog Proteins
Homeodomain Proteins
Limb Buds
Mice, Knockout
Mice, Transgenic
Models, Genetic
Myeloid Ecotropic Viral Integration Site 1 Protein
Neoplasm Proteins
T-Box Domain Proteins
Transcription Factors
Transcriptional Activation
title_short Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.
title_full Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.
title_fullStr Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.
title_full_unstemmed Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.
title_sort Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors.
dc.creator.none.fl_str_mv Delgado, Irene
Giovinazzo, Giovanna
Temiño, Susana
Gauthier, Yves
Balsalobre, Aurelio
Drouin, Jacques
Torres, Miguel
author Delgado, Irene
author_facet Delgado, Irene
Giovinazzo, Giovanna
Temiño, Susana
Gauthier, Yves
Balsalobre, Aurelio
Drouin, Jacques
Torres, Miguel
author_role author
author2 Giovinazzo, Giovanna
Temiño, Susana
Gauthier, Yves
Balsalobre, Aurelio
Drouin, Jacques
Torres, Miguel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Fundación ProCNIC
dc.subject.none.fl_str_mv Animals
Basic Helix-Loop-Helix Transcription Factors
Body Patterning
DNA-Binding Proteins
Fibroblast Growth Factor 10
Hedgehog Proteins
Homeodomain Proteins
Limb Buds
Mice, Knockout
Mice, Transgenic
Models, Genetic
Myeloid Ecotropic Viral Integration Site 1 Protein
Neoplasm Proteins
T-Box Domain Proteins
Transcription Factors
Transcriptional Activation
topic Animals
Basic Helix-Loop-Helix Transcription Factors
Body Patterning
DNA-Binding Proteins
Fibroblast Growth Factor 10
Hedgehog Proteins
Homeodomain Proteins
Limb Buds
Mice, Knockout
Mice, Transgenic
Models, Genetic
Myeloid Ecotropic Viral Integration Site 1 Protein
Neoplasm Proteins
T-Box Domain Proteins
Transcription Factors
Transcriptional Activation
description Meis1 and Meis2 are homeodomain transcription factors that regulate organogenesis through cooperation with Hox proteins. Elimination of Meis genes after limb induction has shown their role in limb proximo-distal patterning; however, limb development in the complete absence of Meis function has not been studied. Here, we report that Meis1/2 inactivation in the lateral plate mesoderm of mouse embryos leads to limb agenesis. Meis and Tbx factors converge in this function, extensively co-binding with Tbx to genomic sites and co-regulating enhancers of Fgf10, a critical factor in limb initiation. Limbs with three deleted Meis alleles show proximal-specific skeletal hypoplasia and agenesis of posterior skeletal elements. This failure in posterior specification results from an early role of Meis factors in establishing the limb antero-posterior prepattern required for Shh activation. Our results demonstrate roles for Meis transcription factors in early limb development and identify their involvement in previously undescribed interaction networks that regulate organogenesis.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-05-25
2021
2021-05-25
2022
2022-12-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/15251
url http://hdl.handle.net/20.500.12105/15251
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404209218060288
score 15,811543

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