Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma.

The mesothelium lines body cavities and surrounds internal organs, widely contributing to homeostasis and regeneration. Mesothelium disruptions cause visceral anomalies and mesothelioma tumors. Nonetheless, the embryonic emergence of mesothelia remains incompletely understood. Here, we track mesothe...

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Detalles Bibliográficos
Autores: Prummel, Karin D, Crowell, Helena L, Nieuwenhuize, Susan, Brombacher, Eline C, Daetwyler, Stephan, Soneson, Charlotte, Kresoja-Rakic, Jelena, Kocere, Agnese, Ronner, Manuel, Ernst, Alexander, Labbaf, Zahra, Clouthier, David E, Firulli, Anthony B, Sanchez-Iranzo, Hector, Naganathan, Sundar R, O'Rourke, Rebecca, Raz, Erez, Mercader, Nadia, Burger, Alexa, Felley-Bosco, Emanuela, Huisken, Jan, Robinson, Mark D, Mosimann, Christian
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/15710
Acceso en línea:http://hdl.handle.net/20.500.12105/15710
Access Level:acceso abierto
Palabra clave:Mesothelioma
Zebrafish
Animals
Basic Helix-Loop-Helix Transcription Factors
Epithelium
Mice
Transcription Factors
Zebrafish Proteins
Descripción
Sumario:The mesothelium lines body cavities and surrounds internal organs, widely contributing to homeostasis and regeneration. Mesothelium disruptions cause visceral anomalies and mesothelioma tumors. Nonetheless, the embryonic emergence of mesothelia remains incompletely understood. Here, we track mesothelial origins in the lateral plate mesoderm (LPM) using zebrafish. Single-cell transcriptomics uncovers a post-gastrulation gene expression signature centered on hand2 in distinct LPM progenitor cells. We map mesothelial progenitors to lateral-most, hand2-expressing LPM and confirm conservation in mouse. Time-lapse imaging of zebrafish hand2 reporter embryos captures mesothelium formation including pericardium, visceral, and parietal peritoneum. We find primordial germ cells migrate with the forming mesothelium as ventral migration boundary. Functionally, hand2 loss disrupts mesothelium formation with reduced progenitor cells and perturbed migration. In mouse and human mesothelioma, we document expression of LPM-associated transcription factors including Hand2, suggesting re-initiation of a developmental program. Our data connects mesothelium development to Hand2, expanding our understanding of mesothelial pathologies.

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