The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived Endolysin

The search for new microbicide compounds is of an urgent need, especially against difficult-to-eradicate biofilm-forming bacteria. One attractive option is the application of cationic multivalent dendrimers as antibacterials and also as carriers of active molecules. These compounds require an adequa...

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Autores: Quintana Sánchez, Sara|||0000-0003-3184-716X, Gómez Casanova, Natalia|||0000-0002-6983-1895, Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285, Gómez Ramírez, Rafael|||0000-0001-6448-2414, Rachuna, Jaroslaw, Wasik, Slawomir, Semaniak, Jacek, Maciejewska, Barbara, Drulis-Kawa, Zuzanna, Ciepluch, Karol, Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935, Arabski, Michal
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/59141
Acceso en línea:http://hdl.handle.net/10017/59141
https://dx.doi.org/10.3390/ijms23031873
Access Level:acceso abierto
Palabra clave:Pseudomonas aeruginosa
carbosilane dendrimers
polyethylene glycol
biofilm
endolysin
bacteriophages
laser interferometry
Química
Chemistry
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spelling The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived EndolysinQuintana Sánchez, Sara|||0000-0003-3184-716XGómez Casanova, Natalia|||0000-0002-6983-1895Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285Gómez Ramírez, Rafael|||0000-0001-6448-2414Rachuna, JaroslawWasik, SlawomirSemaniak, JacekMaciejewska, BarbaraDrulis-Kawa, ZuzannaCiepluch, KarolMata de la Mata, Francisco Javier de la|||0000-0003-0418-3935Arabski, MichalPseudomonas aeruginosacarbosilane dendrimerspolyethylene glycolbiofilmendolysinbacteriophageslaser interferometryQuímicaChemistryThe search for new microbicide compounds is of an urgent need, especially against difficult-to-eradicate biofilm-forming bacteria. One attractive option is the application of cationic multivalent dendrimers as antibacterials and also as carriers of active molecules. These compounds require an adequate hydrophilic/hydrophobic structural balance to maximize the effect. Herein, we evaluated the antimicrobial activity of cationic carbosilane (CBS) dendrimers unmodified or modified with polyethylene glycol (PEG) units, against planktonic and biofilm-forming P. aeruginosa culture. Our study revealed that the presence of PEG destabilized the hydrophilic/hydrophobic balance but reduced the antibacterial activity measured by microbiological cultivation methods, laser interferometry and fluorescence microscopy. On the other hand, the activity can be improved by the combination of the CBS dendrimers with endolysin, a bacteriophage-encoded peptidoglycan hydrolase. This enzyme applied in the absence of the cationic CBS dendrimers is ineffective against Gram-negative bacteria because of the protective outer membrane shield. However, the endolysin-CBS dendrimer mixture enables the penetration through the membrane and then deterioration of the peptidoglycan layer, providing a synergic antimicrobial effectRamón y Cajal Institute of Health Research, IRYCISPolish National Science Center ResearchNetworking Research Center for Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)Ministerio de Economía, Comercio y EmpresaComunidad de MadridUniversidad de Alcalá20222022-02-07journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/59141https://dx.doi.org/10.3390/ijms23031873reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available PID2020-112924RB-I00PNSCR Not available 2017%2F27%2FBUAH Not available EPU-INV%2F2020%2F014Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3703open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/591412026-06-18T11:13:07Z
dc.title.none.fl_str_mv The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
spellingShingle The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
Quintana Sánchez, Sara|||0000-0003-3184-716X
Pseudomonas aeruginosa
carbosilane dendrimers
polyethylene glycol
biofilm
endolysin
bacteriophages
laser interferometry
Química
Chemistry
title_short The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title_full The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title_fullStr The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title_full_unstemmed The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title_sort The Antibacterial Effect of PEGylated Carbosilane Dendrimerson P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
dc.creator.none.fl_str_mv Quintana Sánchez, Sara|||0000-0003-3184-716X
Gómez Casanova, Natalia|||0000-0002-6983-1895
Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285
Gómez Ramírez, Rafael|||0000-0001-6448-2414
Rachuna, Jaroslaw
Wasik, Slawomir
Semaniak, Jacek
Maciejewska, Barbara
Drulis-Kawa, Zuzanna
Ciepluch, Karol
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Arabski, Michal
author Quintana Sánchez, Sara|||0000-0003-3184-716X
author_facet Quintana Sánchez, Sara|||0000-0003-3184-716X
Gómez Casanova, Natalia|||0000-0002-6983-1895
Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285
Gómez Ramírez, Rafael|||0000-0001-6448-2414
Rachuna, Jaroslaw
Wasik, Slawomir
Semaniak, Jacek
Maciejewska, Barbara
Drulis-Kawa, Zuzanna
Ciepluch, Karol
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Arabski, Michal
author_role author
author2 Gómez Casanova, Natalia|||0000-0002-6983-1895
Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285
Gómez Ramírez, Rafael|||0000-0001-6448-2414
Rachuna, Jaroslaw
Wasik, Slawomir
Semaniak, Jacek
Maciejewska, Barbara
Drulis-Kawa, Zuzanna
Ciepluch, Karol
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Arabski, Michal
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pseudomonas aeruginosa
carbosilane dendrimers
polyethylene glycol
biofilm
endolysin
bacteriophages
laser interferometry
Química
Chemistry
topic Pseudomonas aeruginosa
carbosilane dendrimers
polyethylene glycol
biofilm
endolysin
bacteriophages
laser interferometry
Química
Chemistry
description The search for new microbicide compounds is of an urgent need, especially against difficult-to-eradicate biofilm-forming bacteria. One attractive option is the application of cationic multivalent dendrimers as antibacterials and also as carriers of active molecules. These compounds require an adequate hydrophilic/hydrophobic structural balance to maximize the effect. Herein, we evaluated the antimicrobial activity of cationic carbosilane (CBS) dendrimers unmodified or modified with polyethylene glycol (PEG) units, against planktonic and biofilm-forming P. aeruginosa culture. Our study revealed that the presence of PEG destabilized the hydrophilic/hydrophobic balance but reduced the antibacterial activity measured by microbiological cultivation methods, laser interferometry and fluorescence microscopy. On the other hand, the activity can be improved by the combination of the CBS dendrimers with endolysin, a bacteriophage-encoded peptidoglycan hydrolase. This enzyme applied in the absence of the cationic CBS dendrimers is ineffective against Gram-negative bacteria because of the protective outer membrane shield. However, the endolysin-CBS dendrimer mixture enables the penetration through the membrane and then deterioration of the peptidoglycan layer, providing a synergic antimicrobial effect
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-02-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/59141
https://dx.doi.org/10.3390/ijms23031873
url http://hdl.handle.net/10017/59141
https://dx.doi.org/10.3390/ijms23031873
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available PID2020-112924RB-I00
PNSCR Not available 2017%2F27%2FB
UAH Not available EPU-INV%2F2020%2F014
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3703
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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