Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter
The current transition towards the circular bioeconomy requires a rational development of biorefineries to sustainably fulfill the present demands. The use of Komagataella phaffii (Pichia pastoris) can meet this challenge, since it has the capability to use crude glycerol as a carbon-source, a by-pr...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:282622 |
| Acceso en línea: | https://ddd.uab.cat/record/282622 https://dx.doi.org/urn:doi:10.3389/fbioe.2023.1130583 |
| Access Level: | acceso abierto |
| Palabra clave: | Komagataella phaffii PDH Recombinant protein production Methanol-free expression system Growth-decoupled bioprocess Promoter regulation Pichia pastoris |
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Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoterBernat Camps, NúriaEbner, KatharinaSchusterbauer, VeronikaFischer, Jasmin ElginNieto-Taype, Miguel Angel|||0000-0003-1055-0658Valero, Francisco|||0000-0003-0429-9620Glieder, Anton|||0000-0002-5986-9689Garcia-Ortega, Xavier|||0000-0001-7833-3655Komagataella phaffiiPDHRecombinant protein productionMethanol-free expression systemGrowth-decoupled bioprocessPromoter regulationPichia pastorisThe current transition towards the circular bioeconomy requires a rational development of biorefineries to sustainably fulfill the present demands. The use of Komagataella phaffii (Pichia pastoris) can meet this challenge, since it has the capability to use crude glycerol as a carbon-source, a by-product from the biodiesel industry, while producing high- and low-added value products. Recombinant protein production (RPP) using K. phaffii has often been driven either by the methanol induced AOX1 promoter (P) and/or the constitutive GAP promoter (P). In the last years, strong efforts have been focused on developing novel expression systems that expand the toolbox variety of K. phaffii to efficiently produce diverse proteins that requires different strategies. In this work, a study was conducted towards the development of methanol-free expression system based on a heat-shock gene promoter (P) using glycerol as sole carbon source. Using this promoter, the recombinant expression is strongly induced in carbon-starving conditions. The classical P was used as a benchmark, taking for both strains the lipase B from Candida antarctica (CalB) as model protein. Titer of CalB expressed under P outperformed P controlled expression in shake-flask cultivations when using a slow-release continuous feeding technology, confirming that P is induced under pseudo-starving conditions. This increase was also confirmed in fed-batch cultivations. Several optimization rounds were carried out for P under different feeding and osmolarity conditions. In all of them the P controlled process outperformed the P one in regard to CalB titer. The best P approach reached 3.6-fold more specific productivity than P fed-batch at low μ. Compared to the optimum approach for P-based process, the best P fed-batch strategy resulted in 2.3-fold higher titer, while the specific productivity was very similar. To summarize, P is an inducible promoter that exhibited a non-coupled growth regulation showing high performance, which provides a methanol-free additional solution to the usual growth-coupled systems for RPP. Thus, this novel system emerges as a potential alternative for K. phaffii RPP bioprocess and for revaluing crude glycerol, promoting the transition towards a circular economy. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/282622https://dx.doi.org/urn:doi:10.3389/fbioe.2023.1130583reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2826222026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter |
| title |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter |
| spellingShingle |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter Bernat Camps, Núria Komagataella phaffii PDH Recombinant protein production Methanol-free expression system Growth-decoupled bioprocess Promoter regulation Pichia pastoris |
| title_short |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter |
| title_full |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter |
| title_fullStr |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter |
| title_full_unstemmed |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter |
| title_sort |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter |
| dc.creator.none.fl_str_mv |
Bernat Camps, Núria Ebner, Katharina Schusterbauer, Veronika Fischer, Jasmin Elgin Nieto-Taype, Miguel Angel|||0000-0003-1055-0658 Valero, Francisco|||0000-0003-0429-9620 Glieder, Anton|||0000-0002-5986-9689 Garcia-Ortega, Xavier|||0000-0001-7833-3655 |
| author |
Bernat Camps, Núria |
| author_facet |
Bernat Camps, Núria Ebner, Katharina Schusterbauer, Veronika Fischer, Jasmin Elgin Nieto-Taype, Miguel Angel|||0000-0003-1055-0658 Valero, Francisco|||0000-0003-0429-9620 Glieder, Anton|||0000-0002-5986-9689 Garcia-Ortega, Xavier|||0000-0001-7833-3655 |
| author_role |
author |
| author2 |
Ebner, Katharina Schusterbauer, Veronika Fischer, Jasmin Elgin Nieto-Taype, Miguel Angel|||0000-0003-1055-0658 Valero, Francisco|||0000-0003-0429-9620 Glieder, Anton|||0000-0002-5986-9689 Garcia-Ortega, Xavier|||0000-0001-7833-3655 |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Komagataella phaffii PDH Recombinant protein production Methanol-free expression system Growth-decoupled bioprocess Promoter regulation Pichia pastoris |
| topic |
Komagataella phaffii PDH Recombinant protein production Methanol-free expression system Growth-decoupled bioprocess Promoter regulation Pichia pastoris |
| description |
The current transition towards the circular bioeconomy requires a rational development of biorefineries to sustainably fulfill the present demands. The use of Komagataella phaffii (Pichia pastoris) can meet this challenge, since it has the capability to use crude glycerol as a carbon-source, a by-product from the biodiesel industry, while producing high- and low-added value products. Recombinant protein production (RPP) using K. phaffii has often been driven either by the methanol induced AOX1 promoter (P) and/or the constitutive GAP promoter (P). In the last years, strong efforts have been focused on developing novel expression systems that expand the toolbox variety of K. phaffii to efficiently produce diverse proteins that requires different strategies. In this work, a study was conducted towards the development of methanol-free expression system based on a heat-shock gene promoter (P) using glycerol as sole carbon source. Using this promoter, the recombinant expression is strongly induced in carbon-starving conditions. The classical P was used as a benchmark, taking for both strains the lipase B from Candida antarctica (CalB) as model protein. Titer of CalB expressed under P outperformed P controlled expression in shake-flask cultivations when using a slow-release continuous feeding technology, confirming that P is induced under pseudo-starving conditions. This increase was also confirmed in fed-batch cultivations. Several optimization rounds were carried out for P under different feeding and osmolarity conditions. In all of them the P controlled process outperformed the P one in regard to CalB titer. The best P approach reached 3.6-fold more specific productivity than P fed-batch at low μ. Compared to the optimum approach for P-based process, the best P fed-batch strategy resulted in 2.3-fold higher titer, while the specific productivity was very similar. To summarize, P is an inducible promoter that exhibited a non-coupled growth regulation showing high performance, which provides a methanol-free additional solution to the usual growth-coupled systems for RPP. Thus, this novel system emerges as a potential alternative for K. phaffii RPP bioprocess and for revaluing crude glycerol, promoting the transition towards a circular economy. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2 2023-01-01 2023 2023-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/282622 https://dx.doi.org/urn:doi:10.3389/fbioe.2023.1130583 |
| url |
https://ddd.uab.cat/record/282622 https://dx.doi.org/urn:doi:10.3389/fbioe.2023.1130583 |
| dc.language.none.fl_str_mv |
Inglés eng |
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Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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