Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid

Methanol is increasingly gaining attraction as renewable carbon source to produce specialty and commodity chemicals, as it can be generated from renewable sources such as carbon dioxide (CO2). In this context, native methylotrophs such as the yeast Komagataella phaffii (syn Pichia pastoris) are pote...

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Detalles Bibliográficos
Autores: Àvila-Cabré, Sílvia|||0000-0001-6179-4877, Pérez-Trujillo, Míriam|||0000-0002-6919-7417, Albiol i Sala, Joan|||0000-0001-5626-429X, Ferrer, Pau|||0000-0002-5287-4127
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:285891
Acceso en línea:https://ddd.uab.cat/record/285891
https://dx.doi.org/urn:doi:10.1186/s12934-023-02241-9
Access Level:acceso abierto
Palabra clave:3-Hydroxypropionic acid
Pichia pastoris
Komagataella phaffii
Methanol
β-Alanine pathway
Metabolic engineering
Descripción
Sumario:Methanol is increasingly gaining attraction as renewable carbon source to produce specialty and commodity chemicals, as it can be generated from renewable sources such as carbon dioxide (CO2). In this context, native methylotrophs such as the yeast Komagataella phaffii (syn Pichia pastoris) are potentially attractive cell factories to produce a wide range of products from this highly reduced substrate. However, studies addressing the potential of this yeast to produce bulk chemicals from methanol are still scarce. 3-Hydroxypropionic acid (3-HP) is a platform chemical which can be converted into acrylic acid and other commodity chemicals and biopolymers. 3-HP can be naturally produced by several bacteria through different metabolic pathways.