Replication data for research article "Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid"
Supplementary files of research article "Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid". This research paper performs a theoretical comparison between the the two main metabolic pathways towards 3-hydroxypropioni...
| Autores: | , , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Consorci de Serveis Universitaris de Catalunya (CSUC) |
| Repositorio: | CORA.Repositori de Dades de Recerca |
| OAI Identifier: | oai:dnet:cora.rdr____::64ffa6be5380064cf04ca00e3b7ff332 |
| Acceso en línea: | https://doi.org/10.34810/DATA1104 |
| Access Level: | acceso abierto |
| Palabra clave: | Engineering 3-hydroxypropionic acid Pichia pastoris Komagataella phaffii Methanol β-alanine pathway |
| Sumario: | Supplementary files of research article "Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid". This research paper performs a theoretical comparison between the the two main metabolic pathways towards 3-hydroxypropionic (3-HP) acid production, and describes the establishment of the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-HP. Several strains overexpressing the β-alanine pathway were constructed and tested on 24 deep-well plate cultivations containing buffered minimal medium (BMM) with methanol.Three independent transformants from each strain were tested. Final biomass concentration as well as methanol and 3-HP quantification were determined at the end of the cultures. Series of growth kinetics experiments were performed by cultivating a representative clone from each strain in shake-flask cultures using BMM to determine the maximum specific growth rate of the different strains. The best performing strains were further evaluated in aerobic fed-batch reactors following a pre-programmed exponential feeding strategy for controlled growth rate. Offline and online state variables during the fed-batch phase of the bioreactor-scale experiments were monitored and recorded. Nuclear Magnetic Resonance (NMR) spectroscopy was used to perform the exometabolome profiling analyses of the supernatant samples from early, mid, and late methanol-feeding phase. All experiments were carried in duplicates. The files attached herein contain: the diagrams and stoichiometric balances of the malonyl-CoA and β-alanine pathways, the raw data obtained from the 24 deep-well plate cultivations and cell growth kinetics experiments, the NMR spectra for different strains' exometabolome, the raw and processed data obtained from the bioreactor-scale experiments, the description of the molecular cloning materials and methods, and the raw data of the online monitored standard process parameters. |
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