Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells

Periodontal disease (PD) is the result of an infection and chronic inflammation of the gingiva that may lead to its destruction and, in severe cases, alveolar bone and tooth loss. The ultimate goal of periodontal treatment is to achieve periodontal soft and hard tissues regeneration. We previously s...

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Detalles Bibliográficos
Autores: Gomez-Florit, Manuel, Monjo, Marta, Ramis, Joana Maria
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/10614
Acceso en línea:https://hdl.handle.net/20.500.13003/10614
Access Level:acceso abierto
Palabra clave:Cytokines
Regeneration
Osteoblasts
Male
Middle Aged
Gingiva
Periodontium
Cells, Cultured
Humans
Gene Expression Regulation
Cell Differentiation
Fibroblasts
Female
Quercetin
Adult
Young Adult
Interleukin-1beta
Inflammation Mediators
Osteogenesis
Interleucina-1beta
Mediadores de Inflamación
Citocinas
Femenino
Encía
Periodoncio
Masculino
Regulación de la Expresión Génica
Osteoblastos
Diferenciación Celular
Humanos
Persona de Mediana Edad
Adulto Joven
Células Cultivadas
Fibroblastos
Regeneración
Quercetina
Osteogénesis
Adulto
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network_name_str España
repository_id_str
spelling Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cellsGomez-Florit, ManuelMonjo, MartaRamis, Joana MariaCytokinesRegenerationOsteoblastsMaleMiddle AgedGingivaPeriodontiumCells, CulturedHumansGene Expression RegulationCell DifferentiationFibroblastsFemaleQuercetinAdultYoung AdultInterleukin-1betaInflammation MediatorsOsteogenesisInterleucina-1betaMediadores de InflamaciónCitocinasFemeninoEncíaPeriodoncioMasculinoRegulación de la Expresión GénicaOsteoblastosDiferenciación CelularHumanosPersona de Mediana EdadAdulto JovenCélulas CultivadasFibroblastosRegeneraciónQuercetinaOsteogénesisAdultoPeriodontal disease (PD) is the result of an infection and chronic inflammation of the gingiva that may lead to its destruction and, in severe cases, alveolar bone and tooth loss. The ultimate goal of periodontal treatment is to achieve periodontal soft and hard tissues regeneration. We previously selected quercitrin, a catechol-containing flavonoid, as a potential agent for periodontal applications. In this study, we tested the ability of quercitrin to alter biomarker production involved in periodontal regeneration on primary human gingival fibroblasts (hGF) and primary human mesenchymal stem cells (hMSC) cultured under basal and inflammatory conditions. To mimic PD inflammatory status, interleukin-1 beta (IL-1 beta) was used. The expression of different genes related to inflammation and extracellular matrix were evaluated and prostaglandin E2 (PGE2) production was quantified in hGFs; alkaline phosphatase (ALP) activity and calcium content were analysed in hMSCs. Quercitrin decreased the release of the inflammatory mediator PGE2 and partially re-established the impaired collagen metabolism induced by IL-1 beta treatment in hGFs. Quercitrin also increased ALP activity and mineralization in hMSCs, thus, it increased hMSCs differentiation towards the osteoblastic lineage. These findings suggest quercitrin as a novel bioactive molecule with application to enhance both soft and hard tissue regeneration of the periodontium.Nature Publishing Group20152015-11-1220152015-11-12research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/10614reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/106142026-06-22T12:44:07Z
dc.title.none.fl_str_mv Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
title Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
spellingShingle Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
Gomez-Florit, Manuel
Cytokines
Regeneration
Osteoblasts
Male
Middle Aged
Gingiva
Periodontium
Cells, Cultured
Humans
Gene Expression Regulation
Cell Differentiation
Fibroblasts
Female
Quercetin
Adult
Young Adult
Interleukin-1beta
Inflammation Mediators
Osteogenesis
Interleucina-1beta
Mediadores de Inflamación
Citocinas
Femenino
Encía
Periodoncio
Masculino
Regulación de la Expresión Génica
Osteoblastos
Diferenciación Celular
Humanos
Persona de Mediana Edad
Adulto Joven
Células Cultivadas
Fibroblastos
Regeneración
Quercetina
Osteogénesis
Adulto
title_short Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
title_full Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
title_fullStr Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
title_full_unstemmed Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
title_sort Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
dc.creator.none.fl_str_mv Gomez-Florit, Manuel
Monjo, Marta
Ramis, Joana Maria
author Gomez-Florit, Manuel
author_facet Gomez-Florit, Manuel
Monjo, Marta
Ramis, Joana Maria
author_role author
author2 Monjo, Marta
Ramis, Joana Maria
author2_role author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Cytokines
Regeneration
Osteoblasts
Male
Middle Aged
Gingiva
Periodontium
Cells, Cultured
Humans
Gene Expression Regulation
Cell Differentiation
Fibroblasts
Female
Quercetin
Adult
Young Adult
Interleukin-1beta
Inflammation Mediators
Osteogenesis
Interleucina-1beta
Mediadores de Inflamación
Citocinas
Femenino
Encía
Periodoncio
Masculino
Regulación de la Expresión Génica
Osteoblastos
Diferenciación Celular
Humanos
Persona de Mediana Edad
Adulto Joven
Células Cultivadas
Fibroblastos
Regeneración
Quercetina
Osteogénesis
Adulto
topic Cytokines
Regeneration
Osteoblasts
Male
Middle Aged
Gingiva
Periodontium
Cells, Cultured
Humans
Gene Expression Regulation
Cell Differentiation
Fibroblasts
Female
Quercetin
Adult
Young Adult
Interleukin-1beta
Inflammation Mediators
Osteogenesis
Interleucina-1beta
Mediadores de Inflamación
Citocinas
Femenino
Encía
Periodoncio
Masculino
Regulación de la Expresión Génica
Osteoblastos
Diferenciación Celular
Humanos
Persona de Mediana Edad
Adulto Joven
Células Cultivadas
Fibroblastos
Regeneración
Quercetina
Osteogénesis
Adulto
description Periodontal disease (PD) is the result of an infection and chronic inflammation of the gingiva that may lead to its destruction and, in severe cases, alveolar bone and tooth loss. The ultimate goal of periodontal treatment is to achieve periodontal soft and hard tissues regeneration. We previously selected quercitrin, a catechol-containing flavonoid, as a potential agent for periodontal applications. In this study, we tested the ability of quercitrin to alter biomarker production involved in periodontal regeneration on primary human gingival fibroblasts (hGF) and primary human mesenchymal stem cells (hMSC) cultured under basal and inflammatory conditions. To mimic PD inflammatory status, interleukin-1 beta (IL-1 beta) was used. The expression of different genes related to inflammation and extracellular matrix were evaluated and prostaglandin E2 (PGE2) production was quantified in hGFs; alkaline phosphatase (ALP) activity and calcium content were analysed in hMSCs. Quercitrin decreased the release of the inflammatory mediator PGE2 and partially re-established the impaired collagen metabolism induced by IL-1 beta treatment in hGFs. Quercitrin also increased ALP activity and mineralization in hMSCs, thus, it increased hMSCs differentiation towards the osteoblastic lineage. These findings suggest quercitrin as a novel bioactive molecule with application to enhance both soft and hard tissue regeneration of the periodontium.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-11-12
2015
2015-11-12
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/10614
url https://hdl.handle.net/20.500.13003/10614
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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