Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
Periodontal disease (PD) is the result of an infection and chronic inflammation of the gingiva that may lead to its destruction and, in severe cases, alveolar bone and tooth loss. The ultimate goal of periodontal treatment is to achieve periodontal soft and hard tissues regeneration. We previously s...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Conselleria de Salut i Consum del Govern de les Illes Balears |
| Repositorio: | Docusalut |
| Idioma: | inglés |
| OAI Identifier: | oai:docusalut.com:20.500.13003/10614 |
| Acceso en línea: | https://hdl.handle.net/20.500.13003/10614 |
| Access Level: | acceso abierto |
| Palabra clave: | Cytokines Regeneration Osteoblasts Male Middle Aged Gingiva Periodontium Cells, Cultured Humans Gene Expression Regulation Cell Differentiation Fibroblasts Female Quercetin Adult Young Adult Interleukin-1beta Inflammation Mediators Osteogenesis Interleucina-1beta Mediadores de Inflamación Citocinas Femenino Encía Periodoncio Masculino Regulación de la Expresión Génica Osteoblastos Diferenciación Celular Humanos Persona de Mediana Edad Adulto Joven Células Cultivadas Fibroblastos Regeneración Quercetina Osteogénesis Adulto |
| Sumario: | Periodontal disease (PD) is the result of an infection and chronic inflammation of the gingiva that may lead to its destruction and, in severe cases, alveolar bone and tooth loss. The ultimate goal of periodontal treatment is to achieve periodontal soft and hard tissues regeneration. We previously selected quercitrin, a catechol-containing flavonoid, as a potential agent for periodontal applications. In this study, we tested the ability of quercitrin to alter biomarker production involved in periodontal regeneration on primary human gingival fibroblasts (hGF) and primary human mesenchymal stem cells (hMSC) cultured under basal and inflammatory conditions. To mimic PD inflammatory status, interleukin-1 beta (IL-1 beta) was used. The expression of different genes related to inflammation and extracellular matrix were evaluated and prostaglandin E2 (PGE2) production was quantified in hGFs; alkaline phosphatase (ALP) activity and calcium content were analysed in hMSCs. Quercitrin decreased the release of the inflammatory mediator PGE2 and partially re-established the impaired collagen metabolism induced by IL-1 beta treatment in hGFs. Quercitrin also increased ALP activity and mineralization in hMSCs, thus, it increased hMSCs differentiation towards the osteoblastic lineage. These findings suggest quercitrin as a novel bioactive molecule with application to enhance both soft and hard tissue regeneration of the periodontium. |
|---|