Genome-wide phenotypic RNAi screen in the Drosophila wing: Phenotypic description of functional classes

The Drosophila genome contains approximately 14,000 protein-coding genes encoding all the necessary information to sustain cellular physiology, tissue organization, organism development, and behavior. In this manuscript, we describe in some detail the phenotypes in the adult fly wing generated after...

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Detalles Bibliográficos
Autores: López-Varea, Ana, Vega Cuesta, Patricia, Ruiz Gómez, Ana, Ostalé, Cristina M., Molnar, Cristina, Hevia, Covadonga F., Martín, Mercedes, Organista, Maria F., De Celis, Jesús, Culí, Joaquín, Esteban, Nuria, De Celis, Jose F.
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/704566
Acceso en línea:http://hdl.handle.net/10486/704566
https://dx.doi.org/10.1093/g3journal/jkab349
Access Level:acceso abierto
Palabra clave:Drosophila
Drosophila Proteins
Phenotype
RNA Interference
Wings, Animal
Genetics
Metabolism
Biología y Biomedicina / Biología
Descripción
Sumario:The Drosophila genome contains approximately 14,000 protein-coding genes encoding all the necessary information to sustain cellular physiology, tissue organization, organism development, and behavior. In this manuscript, we describe in some detail the phenotypes in the adult fly wing generated after knockdown of approximately 80% of Drosophila genes. We combined this phenotypic description with a comprehensive molecular classification of the Drosophila proteins into classes that summarize the main expected or known biochemical/functional aspect of each protein. This information, combined with mRNA expression levels and in situ expression patterns, provides a simplified atlas of the Drosophila genome, from housekeeping proteins to the components of the signaling pathways directing wing development, that might help to further understand the contribution of each gene group to wing formation