Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac Disease

This article belongs to the Special Issue Advanced Polyphenol Research: Enhancing the Health Benefits of Polyphenols in Plant-Based Foods.

Detalles Bibliográficos
Autores: Girbal, Marina, Rodríguez-Lagunas, María José, Rodríguez-Banqueri, Arturo, Eckhard, Ulrich, Gomis-Rüth, F. Xavier, Franch-Masferrer, Àngels, Pérez-Cano, Francisco J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/417107
Acceso en línea:http://hdl.handle.net/10261/417107
Access Level:acceso abierto
Palabra clave:Celiac disease (CeD)
Polyphenols
Cocoa
Bioactives
PICRUSt2
Microbiota
Functional prediction
Goblet cells
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oai_identifier_str oai:digital.csic.es:10261/417107
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac Disease
title Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac Disease
spellingShingle Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac Disease
Girbal, Marina
Celiac disease (CeD)
Polyphenols
Cocoa
Bioactives
PICRUSt2
Microbiota
Functional prediction
Goblet cells
title_short Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac Disease
title_full Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac Disease
title_fullStr Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac Disease
title_full_unstemmed Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac Disease
title_sort Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac Disease
dc.creator.none.fl_str_mv Girbal, Marina
Rodríguez-Lagunas, María José
Rodríguez-Banqueri, Arturo
Eckhard, Ulrich
Gomis-Rüth, F. Xavier
Franch-Masferrer, Àngels
Pérez-Cano, Francisco J.
author Girbal, Marina
author_facet Girbal, Marina
Rodríguez-Lagunas, María José
Rodríguez-Banqueri, Arturo
Eckhard, Ulrich
Gomis-Rüth, F. Xavier
Franch-Masferrer, Àngels
Pérez-Cano, Francisco J.
author_role author
author2 Rodríguez-Lagunas, María José
Rodríguez-Banqueri, Arturo
Eckhard, Ulrich
Gomis-Rüth, F. Xavier
Franch-Masferrer, Àngels
Pérez-Cano, Francisco J.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Barcelona
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Generalitat de Catalunya
Girbal, Marina [0000-0002-8370-6396]
Rodríguez-Lagunas, María José [0000-0001-7870-8250]
Rodríguez-Banqueri, Arturo [0000-0001-6183-0639]
Eckhard, Ulrich [0000-0001-5863-4514]
Gomis-Rüth, F. Xavier [0000-0002-6848-6874]
Franch-Masferrer, Àngels [0000-0002-2362-1374]
Pérez-Cano, Francisco J. [0000-0001-6243-9164]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Celiac disease (CeD)
Polyphenols
Cocoa
Bioactives
PICRUSt2
Microbiota
Functional prediction
Goblet cells
topic Celiac disease (CeD)
Polyphenols
Cocoa
Bioactives
PICRUSt2
Microbiota
Functional prediction
Goblet cells
description This article belongs to the Special Issue Advanced Polyphenol Research: Enhancing the Health Benefits of Polyphenols in Plant-Based Foods.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/417107
url http://hdl.handle.net/10261/417107
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137827OB-I00
info:eu-repo/grantAgreement/AEI//PID2025-171123OB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/foods15020370
https://doi.org/10.3390/foods15020370
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Cocoa Supplementation Alleviates Gliadin-Induced Intestinal Dysbiosis in a Mouse Model of Celiac DiseaseGirbal, MarinaRodríguez-Lagunas, María JoséRodríguez-Banqueri, ArturoEckhard, UlrichGomis-Rüth, F. XavierFranch-Masferrer, ÀngelsPérez-Cano, Francisco J.Celiac disease (CeD)PolyphenolsCocoaBioactivesPICRUSt2MicrobiotaFunctional predictionGoblet cellsThis article belongs to the Special Issue Advanced Polyphenol Research: Enhancing the Health Benefits of Polyphenols in Plant-Based Foods.Celiac disease (CeD) is a chronic immune-mediated enteropathy triggered by dietary gluten in genetically predisposed individuals which also entails intestinal dysbiosis. This hallmark microbial imbalance provides a rationale for exploring interventions that could modulate the gut ecosystem. Cocoa is a bioactive food rich in polyphenols, theobromine, and fiber, compounds known to have an influence on both immune function and gut microbiota composition. Here, we investigated the effects of cocoa supplementation on the gut microbial profile and predicted functionality in DQ8-Dd-villin-IL-15tg mice, genetically predisposed to CeD. Animals were assigned to a reference group receiving a gluten-free diet (GFD), a gluten-containing diet group (GLI), or the latter supplemented with defatted cocoa (GLI + COCOA) for 25 days. The cecal microbiota was analyzed via 16S rRNA sequencing, and functional pathways were inferred using PICRUSt2. Goblet cell counts and CeD-relevant autoantibodies were measured and correlated with microbial taxa. Cocoa supplementation partially attenuated gluten-induced dysbiosis, preserving beneficial taxa such as Akkermansia muciniphila and Lactobacillus species while reducing opportunistic and pro-inflammatory bacteria. Functional predictions suggested differences in the predicted microbial metabolic potential related to amino acid, vitamin, and phenolic compound metabolism. Cocoa also mitigated goblet cell loss and was inversely associated with anti-gliadin IgA levels. These findings suggest that cocoa, as an adjuvant to a GFD, could be of help in maintaining microbial homeostasis and intestinal health in CeD, supporting further studies to assess its translational potential.M.G.-G. was supported by the predoctoral grant PREDOCS-UB (2022) from the University of Barcelona. U.E. was supported by the Spanish State Research Agency (MCIN/AEI/10.13039/501100011033) through grant RYC2020-029773-I. Additional funding was provided to F.X.G.-R. from the same institution through grants PID2022-137827OB-I00 and PID2025-171123OB-I00. F.X.G.-R and U.E. further received funding from the AGAUR program of the Generalitat de Catalunya (Consolidated Research Group 2021SGR00423).Peer reviewedMultidisciplinary Digital Publishing InstituteUniversidad de BarcelonaAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Generalitat de CatalunyaGirbal, Marina [0000-0002-8370-6396]Rodríguez-Lagunas, María José [0000-0001-7870-8250]Rodríguez-Banqueri, Arturo [0000-0001-6183-0639]Eckhard, Ulrich [0000-0001-5863-4514]Gomis-Rüth, F. Xavier [0000-0002-6848-6874]Franch-Masferrer, Àngels [0000-0002-2362-1374]Pérez-Cano, Francisco J. [0000-0001-6243-9164]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2026202620262026info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/417107reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137827OB-I00info:eu-repo/grantAgreement/AEI//PID2025-171123OB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/foods15020370https://doi.org/10.3390/foods15020370Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4171072026-05-22T06:33:51Z
score 15,811543

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