Meiotic cohesin RAD21L shapes 3D genome structure and transcription in the male germline
[EN]The distinctive three-dimensional (3D) chromatin architecture adopted by chromosomes during meiosis is essential for fertility, yet the functional implications of the fine-scale spatial organization remain poorly understood. Here, we investigate the impact of RAD21L deletion, a meiosis-specific...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/169243 |
| Acceso en línea: | http://hdl.handle.net/10366/169243 |
| Access Level: | acceso abierto |
| Palabra clave: | meiosis cohesin 3D genome chromatin architecture Spermatogenesis Meiosis Spermatocytes Chromatin Spermatogonia Animals Genome Germ Cells Cell Cycle Proteins Mice 2407 Biología Celular cromatina espermatocitos animales espermatogénesis ratones células germinativas genoma espermatogonias proteínas del ciclo celular |
| Sumario: | [EN]The distinctive three-dimensional (3D) chromatin architecture adopted by chromosomes during meiosis is essential for fertility, yet the functional implications of the fine-scale spatial organization remain poorly understood. Here, we investigate the impact of RAD21L deletion, a meiosis-specific cohesin subunit, on the 3D genome architecture and gene expression in the male germ line. Using fluorescence-activated cell sorting, high-throughput chromosome conformation capture, and single-cell RNA sequencing, we demonstrate that RAD21L deficiency impairs meiotic chromatin organization and inter/intrachromosomal interactions. This conspicuous 3D genome reorganization also disrupts bouquet formation, resulting in increased telomeric interactions between heterologous chromosomes in primary spermatocytes. Genome reorganization was accompanied by detectable transcriptional dysregulation in spermatogonia and primary spermatocytes, mainly affecting sex chromosomes. Collectively, our findings establish the cohesin RAD21L as a germline-specific critical regulator of genome-wide 3D genome reorganization during spermatogenesis and a modulator of the male germline transcriptional landscape. |
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