Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi

Salmonella Typhi (S. Typhi), a Gram-negative bacterium, is an intracellular facultative pathogen that causes typhoid fever in humans, and is ingested primarily by water and contaminated food. Once in the intestine, the bacteria invade non-phagocytic cells, such as epithelial cells of the intestinal...

Full description

Bibliographic Details
Author: Castro González, Daniela de los Ángeles
Format: doctoral thesis
Status:Published version
Publication Date:2010
Country:Chile
OAI Identifier:oai:repositorio.anid.cl:10533/232873
Online Access:https://hdl.handle.net/10533/232873
Access Level:Open access
Keyword:Ciencias Naturales
Otras Ciencias Naturales
id CL_3296502c0d5d031c8f107fa276d9a4a1
oai_identifier_str oai:repositorio.anid.cl:10533/232873
network_acronym_str CL
network_name_str Chile
repository_id_str
dc.title.es_CL.fl_str_mv Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi
Characterization of genomic island SPI-8 of Salmonella enterica serovar Typhi
dc.title.none.fl_str_mv Caracterización de la isla genómica spi-8 de salmonella enterica serovar typhi
characterizatión of genomic island spi-8 of salmonella enterica serovar typhi
title Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi
spellingShingle Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi
Castro González, Daniela de los Ángeles
Ciencias Naturales
Otras Ciencias Naturales
title_short Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi
title_full Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi
title_fullStr Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi
title_full_unstemmed Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi
title_sort Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi
dc.creator.none.fl_str_mv Castro González, Daniela de los Ángeles
author Castro González, Daniela de los Ángeles
author_facet Castro González, Daniela de los Ángeles
author_role author
dc.contributor.advisor.none.fl_str_mv Mora, Guido
dc.contributor.institution.es_CL.fl_str_mv PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE
dc.subject.oecd1n.es_CL.fl_str_mv Ciencias Naturales
topic Ciencias Naturales
Otras Ciencias Naturales
dc.subject.oecd2n.es_CL.fl_str_mv Otras Ciencias Naturales
description Salmonella Typhi (S. Typhi), a Gram-negative bacterium, is an intracellular facultative pathogen that causes typhoid fever in humans, and is ingested primarily by water and contaminated food. Once in the intestine, the bacteria invade non-phagocytic cells, such as epithelial cells of the intestinal mucosa; it survives phagocytosis and is also capable of proliferating in infected cells within specialized compartments. Despite the intense study that has been carried out to understand the molecular basis of This disease, the mechanisms that allow this bacterium to infect exclusively humans and higher primates are not yet known. Comparisons between the complete sequences of the genomes of S. Typhi CT18, S. Typhi Ty2 and S. Typhimurium LT2 have allowed the identification of segments of DNA, called Islands of Pathogenicity, that differ from the rest of the genome in several distinctive structural features. The purpose of this work is to study the Salmonella Pathogenicity Island 8 (SPI-8), both in its role in pathogenicity and in its characteristics of unstable element in the genome of Salmonella Typhi. For this, the excision of the island in the genome of S. Typhi, in reference strains and in the collection of clinical isolates STH was evaluated. The possible role of SPI-8 in infection and cell proliferation was also evaluated, evaluating the behavior in human cell lines. With the strategy used and results obtained, it is proposed that SPI-8 is an island of unstable genomics in the way of fixation in the genome
publishDate 2010
dc.date.issued.es_CL.fl_str_mv 2010
dc.date.accessioned.none.fl_str_mv 2019-01-11T12:54:19Z
2022-08-23T12:52:07Z
dc.date.available.none.fl_str_mv 2019-01-11T12:54:19Z
2022-08-23T12:52:07Z
dc.type.none.fl_str_mv Tesis Doctorado
dc.type.driver.es_CL.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.tesis.none.fl_str_mv Tesis
format doctoralThesis
status_str publishedVersion
dc.identifier.folio.es_CL.fl_str_mv 103074
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/232873
identifier_str_mv 103074
url https://hdl.handle.net/10533/232873
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.projectid.es_CL.fl_str_mv info:eu-repo/grantAgreement//103074
info:eu-repo/grantAgreement//23071035
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93488
dc.rights.es_CL.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/publicdomain/zero/1.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/publicdomain/zero/1.0/
bitstream.url.fl_str_mv https://repositorio.anid.cl/bitstreams/8160002a-37e1-473a-8c9d-59c1fdebd770/download
https://repositorio.anid.cl/bitstreams/b1df09b0-abc4-48eb-a5c1-394676cd16d2/download
https://repositorio.anid.cl/bitstreams/6d0ac881-af06-4314-834d-426359f2f470/download
https://repositorio.anid.cl/bitstreams/b8cfa4c3-dcfa-47e4-919b-b55257fb281a/download
https://repositorio.anid.cl/bitstreams/8c43f564-31d5-418a-9cb0-e9ac3bf68a2f/download
https://repositorio.anid.cl/bitstreams/c9bbeaf4-c67d-4b39-af90-5819da90f44f/download
https://repositorio.anid.cl/bitstreams/ea346ec6-bc30-4085-a6c4-dc2e11441810/download
https://repositorio.anid.cl/bitstreams/2b4ec104-4f1e-41bc-8b9d-d3242c6d2ef5/download
bitstream.checksum.fl_str_mv d2117b90602a5222e9ea80e243c19f57
cf7e0094ee7f628ac49a4a5a361db919
0a703d871bf062c5fdc7850b1496693b
593a6e7305c66c56041a9f9e15a649c1
6cbf0f99f8017a49d408f9f1e3ff604e
b1da71b6cf1775902e92b68402050a45
6371f01ff93d430c528c04d430b75550
fbb9c9229703a90e17a561c8525c01c7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
_version_ 1843518641580015616
spelling PONTIFICIA UNIVERSIDAD CATOLICA DE CHILECastro González, Daniela de los Ángeles2010https://hdl.handle.net/10533/232873http://purl.org/coar/access_right/c_abf2Otras Ciencias NaturalesCiencias NaturalesCaracterización de la isla genómica SPI-8 de Salmonella enterica serovar TyphiMora, GuidoPONTIFICIA UNIVERSIDAD CATOLICA DE CHILEChileCastro González, Daniela de los Ángeles2019-01-11T12:54:19Z2022-08-23T12:52:07Z2019-01-11T12:54:19Z2022-08-23T12:52:07Z2010Salmonella Typhi (S. Typhi), a Gram-negative bacterium, is an intracellular facultative pathogen that causes typhoid fever in humans, and is ingested primarily by water and contaminated food. Once in the intestine, the bacteria invade non-phagocytic cells, such as epithelial cells of the intestinal mucosa; it survives phagocytosis and is also capable of proliferating in infected cells within specialized compartments. Despite the intense study that has been carried out to understand the molecular basis of This disease, the mechanisms that allow this bacterium to infect exclusively humans and higher primates are not yet known. Comparisons between the complete sequences of the genomes of S. Typhi CT18, S. Typhi Ty2 and S. Typhimurium LT2 have allowed the identification of segments of DNA, called Islands of Pathogenicity, that differ from the rest of the genome in several distinctive structural features. The purpose of this work is to study the Salmonella Pathogenicity Island 8 (SPI-8), both in its role in pathogenicity and in its characteristics of unstable element in the genome of Salmonella Typhi. For this, the excision of the island in the genome of S. Typhi, in reference strains and in the collection of clinical isolates STH was evaluated. The possible role of SPI-8 in infection and cell proliferation was also evaluated, evaluating the behavior in human cell lines. With the strategy used and results obtained, it is proposed that SPI-8 is an island of unstable genomics in the way of fixation in the genomeSalmonella Typhi (S. Typhi), una bacteria Gram negativo, es un patógeno intracelular facultativo que causa fiebre tifoidea en humanos, y que es ingerida principalmente por agua y alimentos contaminados. Una vez en el intestino, la bacteria invade células no fagocíticas, como las células epiteliales de la mucosa intestinal; sobrevive a la fagocitosis y además es capaz de proliferar en las células infectadas dentro de compartimentos especializados. A pesar del intenso estudio que se ha realizado para comprender las bases moleculares de esta enfermedad, aún no son conocidos los mecanismos que le permiten a esta bacteria infectar exclusivamente al ser humano y a primates superiores. Comparaciones entre las secuencias completas de los genomas de S. Typhi CT18, S. Typhi Ty2 y S. Typhimurium LT2 han permitido identificar segmentos de DNA, llamados Islas de Patogenicidad, que difieren del resto del genoma en varias características estructurales distintivas. El propósito de este trabajo es estudiar la Isla de Patogenicidad 8 de Salmonella (SPI-8), como tanto en su papel en patogenicidad como en sus características de elemento inestable en el genoma de Salmonella Typhi. Para ello, se evaluó la escisión de la isla en el genoma de S. Typhi, en cepas de referencia y de la colección de aislados clínicos STH. También se evaluó el posible papel de SPI-8 en la infección y proliferación celular, evaluándose el comportamiento en líneas celulares humanas. Con la estrategia utilizada y resultados obtenidos, se propone que SPI-8 es una isla de genómica inestable en vías de fijación en el genomaPFCHA-BecasPFCHA-Becas103074https://hdl.handle.net/10533/232873instname: Conicytreponame: Repositorio Digital RI2.0info:eu-repo/grantAgreement//103074info:eu-repo/grantAgreement//23071035info:eu-repo/semantics/dataset/hdl.handle.net/10533/93488info:eu-repo/semantics/openAccesshttp://creativecommons.org/publicdomain/zero/1.0/Ciencias NaturalesOtras Ciencias NaturalesCaracterización de la isla genómica SPI-8 de Salmonella enterica serovar TyphiCharacterization of genomic island SPI-8 of Salmonella enterica serovar TyphiCaracterización de la isla genómica spi-8 de salmonella enterica serovar typhicharacterizatión of genomic island spi-8 of salmonella enterica serovar typhiTesis Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionTesisTesishttps://hdl.handle.net/10533/232873PFCHA-Becas7fa308b9-4299-41bc-b448-27c7e7ebc85bvirtual::60932-17fa308b9-4299-41bc-b448-27c7e7ebc85bvirtual::60932-1ORIGINALTesis Doctorado_Daniela Castro.pdfTesis Doctoradoapplication/pdf4476422https://repositorio.anid.cl/bitstreams/8160002a-37e1-473a-8c9d-59c1fdebd770/downloadd2117b90602a5222e9ea80e243c19f57MD51Portada tesis_Daniela Castro.pdfPortada Tesisapplication/pdf103173https://repositorio.anid.cl/bitstreams/b1df09b0-abc4-48eb-a5c1-394676cd16d2/downloadcf7e0094ee7f628ac49a4a5a361db919MD52CC-LICENSElicense_rdfapplication/octet-stream1089https://repositorio.anid.cl/bitstreams/6d0ac881-af06-4314-834d-426359f2f470/download0a703d871bf062c5fdc7850b1496693bMD53LICENSElicense.txttext/plain1779https://repositorio.anid.cl/bitstreams/b8cfa4c3-dcfa-47e4-919b-b55257fb281a/download593a6e7305c66c56041a9f9e15a649c1MD54TEXTTesis Doctorado_Daniela Castro.pdf.txtExtracted texttext/plain165387https://repositorio.anid.cl/bitstreams/8c43f564-31d5-418a-9cb0-e9ac3bf68a2f/download6cbf0f99f8017a49d408f9f1e3ff604eMD55Portada tesis_Daniela Castro.pdf.txtExtracted texttext/plain2715https://repositorio.anid.cl/bitstreams/c9bbeaf4-c67d-4b39-af90-5819da90f44f/downloadb1da71b6cf1775902e92b68402050a45MD56THUMBNAILTesis Doctorado_Daniela Castro.pdf.jpgIM Thumbnailimage/jpeg350https://repositorio.anid.cl/bitstreams/ea346ec6-bc30-4085-a6c4-dc2e11441810/download6371f01ff93d430c528c04d430b75550MD57Portada tesis_Daniela Castro.pdf.jpgIM Thumbnailimage/jpeg1274https://repositorio.anid.cl/bitstreams/2b4ec104-4f1e-41bc-8b9d-d3242c6d2ef5/downloadfbb9c9229703a90e17a561c8525c01c7MD5810533/232873oai:repositorio.anid.cl:10533/2328732023-07-24 14:27:01.758http://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccesshttps://repositorio.anid.clRepositorio ANIDaletelier@anid.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
score 15,812429

Similar Items