Caracterización de la isla genómica SPI-8 de Salmonella enterica serovar Typhi
Salmonella Typhi (S. Typhi), a Gram-negative bacterium, is an intracellular facultative pathogen that causes typhoid fever in humans, and is ingested primarily by water and contaminated food. Once in the intestine, the bacteria invade non-phagocytic cells, such as epithelial cells of the intestinal...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | Chile |
| OAI Identifier: | oai:repositorio.anid.cl:10533/232873 |
| Acceso en línea: | https://hdl.handle.net/10533/232873 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciencias Naturales Otras Ciencias Naturales |
| Sumario: | Salmonella Typhi (S. Typhi), a Gram-negative bacterium, is an intracellular facultative pathogen that causes typhoid fever in humans, and is ingested primarily by water and contaminated food. Once in the intestine, the bacteria invade non-phagocytic cells, such as epithelial cells of the intestinal mucosa; it survives phagocytosis and is also capable of proliferating in infected cells within specialized compartments. Despite the intense study that has been carried out to understand the molecular basis of This disease, the mechanisms that allow this bacterium to infect exclusively humans and higher primates are not yet known. Comparisons between the complete sequences of the genomes of S. Typhi CT18, S. Typhi Ty2 and S. Typhimurium LT2 have allowed the identification of segments of DNA, called Islands of Pathogenicity, that differ from the rest of the genome in several distinctive structural features. The purpose of this work is to study the Salmonella Pathogenicity Island 8 (SPI-8), both in its role in pathogenicity and in its characteristics of unstable element in the genome of Salmonella Typhi. For this, the excision of the island in the genome of S. Typhi, in reference strains and in the collection of clinical isolates STH was evaluated. The possible role of SPI-8 in infection and cell proliferation was also evaluated, evaluating the behavior in human cell lines. With the strategy used and results obtained, it is proposed that SPI-8 is an island of unstable genomics in the way of fixation in the genome |
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