Allogenic platelet-rich plasma in induced ulcers in rat’s cornea

The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety...

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Bibliographic Details
Authors: Gandolfi, M.g. [UNESP], Brandão, C.v.s. [UNESP], Pellizon, C.h. [UNESP], Hirota, I.n. [UNESP], Barros, R. [UNESP], Hussein, A.c.b. [UNESP], Merlini, N.b. [UNESP], Takahira, R.k. [UNESP], Mesquita, L.r. [UNESP]
Format: article
Status:Published version
Publication Date:2021
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:English
OAI Identifier:oai:repositorio.unesp.br:11449/212667
Online Access:http://dx.doi.org/10.1590/1678-4162-12214
http://hdl.handle.net/11449/212667
Access Level:Open access
Keyword:heated platelet-rich plasma
growth factor
PDGF
plasma rich in growth factors
eye drops
plasma rico em plaquetas aquecido
fator de crescimento
plasma rico em fatores de crescimento
colírios
Description
Summary:The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments.

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