Allogenic platelet-rich plasma in induced ulcers in rat’s cornea

The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety...

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Detalles Bibliográficos
Autores: Gandolfi, M.g. [UNESP], Brandão, C.v.s. [UNESP], Pellizon, C.h. [UNESP], Hirota, I.n. [UNESP], Barros, R. [UNESP], Hussein, A.c.b. [UNESP], Merlini, N.b. [UNESP], Takahira, R.k. [UNESP], Mesquita, L.r. [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/212667
Acceso en línea:http://dx.doi.org/10.1590/1678-4162-12214
http://hdl.handle.net/11449/212667
Access Level:acceso abierto
Palabra clave:heated platelet-rich plasma
growth factor
PDGF
plasma rich in growth factors
eye drops
plasma rico em plaquetas aquecido
fator de crescimento
plasma rico em fatores de crescimento
colírios
Descripción
Sumario:The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments.

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