High Retention and Purification of Bromelain Enzyme (Ananas comosus L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques

The demand for bromelian and pineapple fruit has been increasing substantially in the world because of their benefits for the human health and use in diverse areas. In this context, this work aimed to study the capacity of higher retention (concentration); bromelain activity underwent ultrafiltratio...

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Detalles Bibliográficos
Autores: Gamarra, Felix M. Carbajal, Santana, José C. C., Llanos, Segundo A. V., Pérez, Jorge A. Heredia, Flausino, Fábio Richard, Quispe, Ada P. B., Mendoza, Pedro Córdova, Vanalle, Rosangela M., Carreño-Farfan, Carmen, Berssaneti, Fernando T., de Souza, Roberto R., Tambourgi, Elias B.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/223246
Acceso en línea:http://dx.doi.org/10.3390/polym14020264
http://hdl.handle.net/11449/223246
Access Level:acceso abierto
Palabra clave:Bromelain
Enzymatic activity
Hollow fiber membrane
Pineapple
Plain membrane
Separation process
Descripción
Sumario:The demand for bromelian and pineapple fruit has been increasing substantially in the world because of their benefits for the human health and use in diverse areas. In this context, this work aimed to study the capacity of higher retention (concentration); bromelain activity underwent ultrafiltration from pineapple juice (Ananas comusus L. Merrill). All assays were carried out at pH 7.0 and 7.5, and at 0.05 and 0.40 bar of transmembrane pressures. Results have shown that at the best operating conditions, between 85 and 87% of bromelain activity was recovered using the plain membrane separation process at 0.05 bar. The ultrafiltration has shown the capacity to retain 100% of proteolytic activity of the bromelain extracted. The samples have kept the same physics properties after ultrafiltration, and the result was verified via electrophoresis. The bromelain enzyme obtained was characterized, and pH 7 and between 30 and 40◦ C were the best conditions. Therefore, this work shows that the use of both polymeric membranes has shown high efficiency, and can be used in the purification of bromelain enzymes.

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