In vitro effects of citral on trypanosoma cruzi metacyclogenesis

Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.

Detalles Bibliográficos
Autores: Cardoso, Josiane, Soares, Maurilio José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/40107
Acceso en línea:https://arca.fiocruz.br/handle/icict/40107
Access Level:acceso abierto
Palabra clave:Citral
Trypanosoma cruzi
Citronela
Cymbopogon
Oils Volatile
Trypanocidal Agents
Aceites Volátiles
Tripanocidas
Óleos Voláteis
Tripanossomicidas
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dc.title.none.fl_str_mv In vitro effects of citral on trypanosoma cruzi metacyclogenesis
title In vitro effects of citral on trypanosoma cruzi metacyclogenesis
spellingShingle In vitro effects of citral on trypanosoma cruzi metacyclogenesis
Cardoso, Josiane
Citral
Trypanosoma cruzi
Citronela
Cymbopogon
Oils Volatile
Trypanocidal Agents
Aceites Volátiles
Tripanocidas
Óleos Voláteis
Tripanossomicidas
title_short In vitro effects of citral on trypanosoma cruzi metacyclogenesis
title_full In vitro effects of citral on trypanosoma cruzi metacyclogenesis
title_fullStr In vitro effects of citral on trypanosoma cruzi metacyclogenesis
title_full_unstemmed In vitro effects of citral on trypanosoma cruzi metacyclogenesis
title_sort In vitro effects of citral on trypanosoma cruzi metacyclogenesis
dc.creator.none.fl_str_mv Cardoso, Josiane
Soares, Maurilio José
author Cardoso, Josiane
author_facet Cardoso, Josiane
Soares, Maurilio José
author_role author
author2 Soares, Maurilio José
author2_role author
dc.subject.other.none.fl_str_mv Citral
Trypanosoma cruzi
Citronela
topic Citral
Trypanosoma cruzi
Citronela
Cymbopogon
Oils Volatile
Trypanocidal Agents
Aceites Volátiles
Tripanocidas
Óleos Voláteis
Tripanossomicidas
dc.subject.en.none.fl_str_mv Cymbopogon
Oils Volatile
Trypanocidal Agents
dc.subject.es.none.fl_str_mv Aceites Volátiles
Tripanocidas
dc.subject.decs.none.fl_str_mv Óleos Voláteis
Tripanossomicidas
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2020-02-20T15:54:15Z
dc.date.available.fl_str_mv 2020-02-20T15:54:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARDOSO, Josiane; SOARES, Maurilio José. In vitro effects of citral on Trypanosoma cruzi metacyclogenesis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 8, p. 1026-1032, 2010.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/40107
dc.identifier.issn.none.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762010000800012
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv CARDOSO, Josiane; SOARES, Maurilio José. In vitro effects of citral on Trypanosoma cruzi metacyclogenesis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 8, p. 1026-1032, 2010.
1678-8060
10.1590/s0074-02762010000800012
url https://arca.fiocruz.br/handle/icict/40107
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fiocruz/IOC
publisher.none.fl_str_mv Fiocruz/IOC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2020-02-20T15:54:15Z2020-02-20T15:54:15Z2010CARDOSO, Josiane; SOARES, Maurilio José. In vitro effects of citral on Trypanosoma cruzi metacyclogenesis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 8, p. 1026-1032, 2010.1678-8060https://arca.fiocruz.br/handle/icict/4010710.1590/s0074-027620100008000121678-8060engFiocruz/IOCCitralTrypanosoma cruziCitronelaCymbopogonOils VolatileTrypanocidal AgentsAceites VolátilesTripanocidasÓleos VoláteisTripanossomicidasIn vitro effects of citral on trypanosoma cruzi metacyclogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Citral, the main constituent of lemongrass (Cymbopogon citratus) essential oil, was added to Trypanosoma cruzi cultures grown in TAU3AAG medium to observe the effect on the epimastigote-to-trypomastigote differentiation process (metacyclogenesis). Our results showed that citral (20 μg/mL) did not affect epimastigote viability or inhibit the differentiation process. Concentrations higher than 60 μg/mL, however, led to 100% cell death (both epimastigote and trypomastigote forms). Although epimastigotes incubated with 30 μg/mL citral were viable and able to adhere to the substrate, we observed around 50% inhibition in metacyclogenesis, with a calculated concentration that inhibited metacyclogenesis by 50% after 24 h (IC50/24 h) of about 31 μg/mL. Treatment with 30 μg/mL citral did not hinder epimastigote multiplication because epimastigote growth resumed when treated cells were transferred to a drug-free liver infusion tryptose culture medium. Metacyclogenesis was almost totally abolished at 40 μg/mL after 24 h of incubation. Furthermore, the metacyclic trypomastigotes obtained in vitro were similarly susceptible to citral, with an IC50/24 h, concentration that killed 50% of the cells after 24 h, of about 24.5 μg/mL. Therefore, citral appears to be a good candidate as an inhibitory drug for further studies analyzing the T. cruzi metacyclogenesis process.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZCardoso, JosianeSoares, Maurilio JoséLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://arca.fiocruz.br/bitstreams/b5a1bed1-b3f6-43b6-b225-6312e2fa390d/download783568c2893d2e25a99990b126be1772MD51falseAnonymousREADORIGINAL12ok.pdf12ok.pdfapplication/pdf506897https://arca.fiocruz.br/bitstreams/df89ee59-bd92-43f7-9923-655cd2f4959e/download2979924cbf48e61c64683658c3506d24MD52trueAnonymousREADTEXT12ok.pdf.txt12ok.pdf.txtExtracted texttext/plain36098https://arca.fiocruz.br/bitstreams/6089033c-cd3c-4761-ad7d-eb6e445e95ba/download52b72624c1e0dd919768808917eaf106MD57falseAnonymousREADTHUMBNAIL12ok.pdf.jpg12ok.pdf.jpgGenerated Thumbnailimage/jpeg46223https://arca.fiocruz.br/bitstreams/1adf3c99-2c5b-499c-9973-e1019b54683d/download7178752e40bc442781cc8fad597842b3MD58falseAnonymousREADicict/401072025-12-10 13:48:06.785open.accessoai:arca.fiocruz.br:icict/40107https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T16:48:06Repositório Institucional da FIOCRUZ (ARCA) - 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