An optimized DNA extraction protocol for benthic Didymosphenia geminata
Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously...
| Authors: | , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2014 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/35693 |
| Online Access: | http://hdl.handle.net/11336/35693 |
| Access Level: | Open access |
| Keyword: | Didymosphenia Dna Extraction Pcr Stalk Benthic Invasive https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Summary: | Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques. |
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