An optimized DNA extraction protocol for benthic Didymosphenia geminata
Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/35693 |
| Acceso en línea: | http://hdl.handle.net/11336/35693 |
| Access Level: | acceso abierto |
| Palabra clave: | Didymosphenia Dna Extraction Pcr Stalk Benthic Invasive https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques. |
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