Effect of hyperandrogenism on ovarian function

The objective of the present work was to study the ovarian function when follicular development is induced during a hyperandrogenic condition. Female rats were injected either with chorionic gonadotropin (eCG group) to induce folliculogenesis or with eCG together with dehydroepiandrosterone to induc...

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Autores: Velez, Leandro Martin, Heber, María Florencia, Ferreira, Silvana Rocio, Abruzzese, Giselle Adriana, Reynoso, Roxana M., Motta, Alicia Beatriz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/13728
Acceso en línea:http://hdl.handle.net/11336/13728
Access Level:acceso abierto
Palabra clave:Hyperandrogenism
Ovary
Ppargamma
Folliculogenesis
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
id AR_590f4488b68f515739fc00a5e427a3ec
oai_identifier_str oai:ri.conicet.gov.ar:11336/13728
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Effect of hyperandrogenism on ovarian function
title Effect of hyperandrogenism on ovarian function
spellingShingle Effect of hyperandrogenism on ovarian function
Velez, Leandro Martin
Hyperandrogenism
Ovary
Ppargamma
Folliculogenesis
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Effect of hyperandrogenism on ovarian function
title_full Effect of hyperandrogenism on ovarian function
title_fullStr Effect of hyperandrogenism on ovarian function
title_full_unstemmed Effect of hyperandrogenism on ovarian function
title_sort Effect of hyperandrogenism on ovarian function
dc.creator.none.fl_str_mv Velez, Leandro Martin
Heber, María Florencia
Ferreira, Silvana Rocio
Abruzzese, Giselle Adriana
Reynoso, Roxana M.
Motta, Alicia Beatriz
author Velez, Leandro Martin
author_facet Velez, Leandro Martin
Heber, María Florencia
Ferreira, Silvana Rocio
Abruzzese, Giselle Adriana
Reynoso, Roxana M.
Motta, Alicia Beatriz
author_role author
author2 Heber, María Florencia
Ferreira, Silvana Rocio
Abruzzese, Giselle Adriana
Reynoso, Roxana M.
Motta, Alicia Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hyperandrogenism
Ovary
Ppargamma
Folliculogenesis
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Hyperandrogenism
Ovary
Ppargamma
Folliculogenesis
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description The objective of the present work was to study the ovarian function when follicular development is induced during a hyperandrogenic condition. Female rats were injected either with chorionic gonadotropin (eCG group) to induce folliculogenesis or with eCG together with dehydroepiandrosterone to induce folliculogenesis in a hyperandrogenic condition (eCG+HA group). The control group was injected with vehicle. Ovarian mRNA levels of the PPARγ co-activator PGC1-α, the PPARγ co-repressor NCoR, and the main enzymes involved in the ovarian steroidogenesis (CYP17, 3β hydroxysteroid dehydrogenase (3β-HSD), 17β hydroxysteroid dehydrogenase (17β-HSD) CYP19A), and cyclooxygenase 2 (COX-2) were evaluated by real time polymerase chain reaction and protein expression of COX-2 was evaluated by Western Blotting. Ovarian steroidogenesis and both the oxidative and inflammatory status were also quantified. We found that eCG-induced folliculogenesis induced increased mRNA levels of PGC1-α and decreased those of NCoR as compared to controls. In addition, we found accumulation of estradiol and enhanced mRNA expression of CYP19A. A pro-inflammatory and a pro-oxidant status were also established. When folliculogenesis was induced in a hyperandrogenic condition, the mRNA levels of the PPARγ co-repressor NCoR remained higher than in controls and the pro-inflammatory and pro-oxidant status were enhanced. In addition, the enzymes involved in ovarian steroidogenesis were altered leading to the accumulation of testosterone and an unfavorable estradiol/testosterone ratio. These alterations led to abnormal follicular development.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13728
Velez, Leandro Martin; Heber, María Florencia; Ferreira, Silvana Rocio; Abruzzese, Giselle Adriana; Reynoso, Roxana M.; et al.; Effect of hyperandrogenism on ovarian function; BioScientifica; Reproduction; 149; 6; 6-2015; 577-585
1470-1626
1741-7899
url http://hdl.handle.net/11336/13728
identifier_str_mv Velez, Leandro Martin; Heber, María Florencia; Ferreira, Silvana Rocio; Abruzzese, Giselle Adriana; Reynoso, Roxana M.; et al.; Effect of hyperandrogenism on ovarian function; BioScientifica; Reproduction; 149; 6; 6-2015; 577-585
1470-1626
1741-7899
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/149/6/577.long
info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-15-0041
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195355429994496
spelling Effect of hyperandrogenism on ovarian functionVelez, Leandro MartinHeber, María FlorenciaFerreira, Silvana RocioAbruzzese, Giselle AdrianaReynoso, Roxana M.Motta, Alicia BeatrizHyperandrogenismOvaryPpargammaFolliculogenesishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The objective of the present work was to study the ovarian function when follicular development is induced during a hyperandrogenic condition. Female rats were injected either with chorionic gonadotropin (eCG group) to induce folliculogenesis or with eCG together with dehydroepiandrosterone to induce folliculogenesis in a hyperandrogenic condition (eCG+HA group). The control group was injected with vehicle. Ovarian mRNA levels of the PPARγ co-activator PGC1-α, the PPARγ co-repressor NCoR, and the main enzymes involved in the ovarian steroidogenesis (CYP17, 3β hydroxysteroid dehydrogenase (3β-HSD), 17β hydroxysteroid dehydrogenase (17β-HSD) CYP19A), and cyclooxygenase 2 (COX-2) were evaluated by real time polymerase chain reaction and protein expression of COX-2 was evaluated by Western Blotting. Ovarian steroidogenesis and both the oxidative and inflammatory status were also quantified. We found that eCG-induced folliculogenesis induced increased mRNA levels of PGC1-α and decreased those of NCoR as compared to controls. In addition, we found accumulation of estradiol and enhanced mRNA expression of CYP19A. A pro-inflammatory and a pro-oxidant status were also established. When folliculogenesis was induced in a hyperandrogenic condition, the mRNA levels of the PPARγ co-repressor NCoR remained higher than in controls and the pro-inflammatory and pro-oxidant status were enhanced. In addition, the enzymes involved in ovarian steroidogenesis were altered leading to the accumulation of testosterone and an unfavorable estradiol/testosterone ratio. These alterations led to abnormal follicular development.Fil: Velez, Leandro Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Heber, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Ferreira, Silvana Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Abruzzese, Giselle Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Reynoso, Roxana M.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Endocrinología; ArgentinaFil: Motta, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaBioScientifica2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13728Velez, Leandro Martin; Heber, María Florencia; Ferreira, Silvana Rocio; Abruzzese, Giselle Adriana; Reynoso, Roxana M.; et al.; Effect of hyperandrogenism on ovarian function; BioScientifica; Reproduction; 149; 6; 6-2015; 577-5851470-16261741-7899enginfo:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/149/6/577.longinfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-15-0041info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:50:00Zoai:ri.conicet.gov.ar:11336/13728instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:50:00.761CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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