Effect of hyperandrogenism on ovarian function
The objective of the present work was to study the ovarian function when follicular development is induced during a hyperandrogenic condition. Female rats were injected either with chorionic gonadotropin (eCG group) to induce folliculogenesis or with eCG together with dehydroepiandrosterone to induc...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/13728 |
| Acceso en línea: | http://hdl.handle.net/11336/13728 |
| Access Level: | acceso abierto |
| Palabra clave: | Hyperandrogenism Ovary Ppargamma Folliculogenesis https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| Sumario: | The objective of the present work was to study the ovarian function when follicular development is induced during a hyperandrogenic condition. Female rats were injected either with chorionic gonadotropin (eCG group) to induce folliculogenesis or with eCG together with dehydroepiandrosterone to induce folliculogenesis in a hyperandrogenic condition (eCG+HA group). The control group was injected with vehicle. Ovarian mRNA levels of the PPARγ co-activator PGC1-α, the PPARγ co-repressor NCoR, and the main enzymes involved in the ovarian steroidogenesis (CYP17, 3β hydroxysteroid dehydrogenase (3β-HSD), 17β hydroxysteroid dehydrogenase (17β-HSD) CYP19A), and cyclooxygenase 2 (COX-2) were evaluated by real time polymerase chain reaction and protein expression of COX-2 was evaluated by Western Blotting. Ovarian steroidogenesis and both the oxidative and inflammatory status were also quantified. We found that eCG-induced folliculogenesis induced increased mRNA levels of PGC1-α and decreased those of NCoR as compared to controls. In addition, we found accumulation of estradiol and enhanced mRNA expression of CYP19A. A pro-inflammatory and a pro-oxidant status were also established. When folliculogenesis was induced in a hyperandrogenic condition, the mRNA levels of the PPARγ co-repressor NCoR remained higher than in controls and the pro-inflammatory and pro-oxidant status were enhanced. In addition, the enzymes involved in ovarian steroidogenesis were altered leading to the accumulation of testosterone and an unfavorable estradiol/testosterone ratio. These alterations led to abnormal follicular development. |
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