A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic

A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Mont...

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Detalles Bibliográficos
Autores: Ramos, Natalia, Panzera Crespo, Yanina, Frabasile Giurato, Sandra Alicia, Tomás Custodio, Gonzalo Martín, Calleros Basilio, Lucía, Marandino, Ana, Techera, Claudia, Grecco Patiño, Sofía, Fuques Villalba, Eddie, Arbiza, Juan, Pérez Crossa, Ruben Gustavo, Delfraro Vázquez, Adriana Beatriz, Goñi Mazzitelli, Natalia, Coppola, Leticia, Ramas, Vivivana, Morel, Noelia, Chiparelli, Héctor
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Uruguay
Institución:Universidad de la República
Repositorio:COLIBRI
Idioma:inglés
OAI Identifier:oai:colibri.udelar.edu.uy:20.500.12008/42441
Acceso en línea:https://hdl.handle.net/20.500.12008/42441
Access Level:acceso abierto
Palabra clave:COVID-19 pandemic
SARS-CoV-2
Human respiratory RNA viruses
Multiplex PCR-NGS
Viral coinfections
Descripción
Sumario:A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts.

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