Efecto de los diluyentes Ley-Leygo y Triladyl y de dos tiempos de equilibrio en la congelación de semen de verraco

The aim of this study was to evaluate the effect of Ley-Leygo and Triladyl® extenders and two equilibrium times on individual motility (IM), plasma membrane integrity (PMI), mitochondrial activity (MA) and intact acrosome (IA) in frozen-thawed boar semen. A rich fraction of a total of 20 ejaculates...

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Bibliographic Details
Authors: Reyes-González, Erika Abigail, Centurión-Castro, Fernando, Uc-Espinosa, Nayeli, Ek-Mex, Jesús Enrique, Peralta-Torres, Jorge Alonso, Segura-Correa, José Candelario
Format: article
Status:Published version
Publication Date:2022
Country:Perú
Institution:Universidad Nacional Mayor de San Marcos
Repository:Revistas - Universidad Nacional Mayor de San Marcos
Language:Spanish
OAI Identifier:oai:revistasinvestigacion.unmsm.edu.pe:article/22758
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/22758
Access Level:Open access
Keyword:criopreservación
semen congelado
reproducción
eyaculado
cryopreservation
frozen semen
reproduction
ejaculate
Description
Summary:The aim of this study was to evaluate the effect of Ley-Leygo and Triladyl® extenders and two equilibrium times on individual motility (IM), plasma membrane integrity (PMI), mitochondrial activity (MA) and intact acrosome (IA) in frozen-thawed boar semen. A rich fraction of a total of 20 ejaculates from five boars of a commercial line was used. All ejaculates had a minimum of 80% motility and 85% normal spermatozoa. Each ejaculate was divided into four aliquots: Ley-Leygo and Triladyl® extenders and two equilibrium times (0 and 1 h). The semen samples were thawed at 37 °C for 20 s. The results of the IM and the PMI did not have significant effects for the factors evaluated (p>0.05). There was a significant difference for MA (p<0.05) between the Ley-Leygo (43.57%) and Triladyl® (51.7%) diluents. The IA was 46% for Ley-Leygo and 25% for Triladyl® (p<0.05). The percentage of IA with the equilibration time of 1 h was greater than that of 0 h (p<0.05). It is concluded that the Triladyl® diluent improved the MA and the equilibration time of 1 h improved the percentage of IA.

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