L-carnitine added to post-thawed semen acts as an antioxidant and a stimulator of equine sperm metabolism

The objective of this study was to enhance the in vitro sperm quality and in vivo fertil-ity of frozen-thawed equine semen by the addition of l-carnitine (LC) to post-thawedsemen. Different concentrations of LC were added to thawed samples to obtain fourtreatments control and 0.5, 1 and 2 mM LC. In...

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Detalhes bibliográficos
Autores: Monique de Albuquerque Lagares, Adalgiza Souza Carneiro de Rezende, Marina Morra Freitas, Rubens Stahlberg, Rafael Romero Nicolino, Grazielle Caroline da Silva, Steyner Franca Cortes, Fabio Henrique Marques Moreira, Franciele Caroline Dias Neves, Natália de Castro Alves, Rodrigo Novaes Viegas, Thiago Frederico Diniz, Virginia Soares Lemos
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:Brasil
Recursos:Universidade Federal de Minas Gerais (UFMG)
Repositório:Repositório Institucional da UFMG
Idioma:português
OAI Identifier:oai:repositorio.ufmg.br:1843/82551
Acesso em linha:http://hdl.handle.net/1843/82551
Access Level:Acceso aberto
Palavra-chave:Stallion
Reproduction
Cryopreservation
Frozen semen
Fertility
Equino
Sêmen congelado
Descrição
Resumo:The objective of this study was to enhance the in vitro sperm quality and in vivo fertil-ity of frozen-thawed equine semen by the addition of l-carnitine (LC) to post-thawedsemen. Different concentrations of LC were added to thawed samples to obtain fourtreatments control and 0.5, 1 and 2 mM LC. In the in vitro experiments, sperm motil-ity and kinematics, membrane integrity and intracellular calcium ion concentration([Ca 2+] i ) were investigated, and the antioxidant bioactivity of LC was assessed bymeasuring hydrogen peroxide and nitrite concentrations (NO2−). The fertility rate wasassessed via the artificial insemination of mares. The treatment with 1 mM LC in-creased sperm [Ca 2+] i (60.6 ± 0.05 AU), reduced nitrite concentration (39.1 ± 14.9 µM/µg protein), increased the sperm straightness percentage (STR: 78.3 ± 5.3%) and in-creased the pregnancy rate (75%) as compared to the control ([Ca 2+] i 48.4 ± 0.05 AU,NO2− concentration 63.1 ± 14.4 µM/µg protein, STR 67.5 ± 7.9%, 12.5% pregnancyrate, p < 0.05). These results suggest that 1 mM LC acts as an antioxidant and stimula-tor of sperm metabolism in post-thawed equine semen, increasing the fertility rate.Thus, addition of LC might be an alternative to improve the fertility of poor qualitypost-thawed equine semen.

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