Morphological evaluation of canine oocytes recovered in different phases of the estrous cycle

The aim of the study was to obtain and morphologically evaluate canine oocytes from females at different phases of the estrous cycle and to compare the proportions of oocytes potentially suitable for in vitro maturation (IVM). Ovaries of 24 bitches, 6 for each phase of the estrous cycle, were sliced...

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Detalhes bibliográficos
Autores: Sánchez R., Alfonso, Ahumada C., Carolina
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Perú
Recursos:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Idioma:español
OAI Identifier:oai:revistasinvestigacion.unmsm.edu.pe:article/11181
Acesso em linha:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11181
Access Level:acceso abierto
Palavra-chave:canine oocyte
in vitro maduration
ovocito canino
maduración in vitro
Descrição
Resumo:The aim of the study was to obtain and morphologically evaluate canine oocytes from females at different phases of the estrous cycle and to compare the proportions of oocytes potentially suitable for in vitro maturation (IVM). Ovaries of 24 bitches, 6 for each phase of the estrous cycle, were sliced to recover the oocytes. These were morphologically classified into fit and unfit oocytes for IVM. The largest proportion of oocytes fit for IVM (86.6%) was recovered from females in proestrus and estrus (p<0.01) and the largest quantity of oocytes fit for IVM per female (448.0 ± 52.7) was obtained in the estrus phase (p<0.01). It is concluded that the morphological assessment of oocytes indicated that the quality and quantity of oocytes recovered during the oestrus and proestrus phase of the oestrus cycle in terms of their potential use form IVM were better and higher than those obtained during the anestrus and diestrus phases.