Design of plasmonic gold nanoantennas and their evaluation for photothermal therapy
"Anisotropic gold nanoparticles (AuNPs) are known as promising tools for localized photo-thermal therapy (PTT) of malignant cells. These anisotropic AuNPs present tunable surface plasmon resonances (SPR) with ideal NIR optical response to be applied as theranostic agents (nanoantennas systems)....
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| Formato: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | México |
| Recursos: | Instituto Potosino de Investigación Científica y Tecnológica |
| Repositorio: | Repositorio Institucional del IPICYT |
| OAI Identifier: | oai:ipicyt.repositorioinstitucional.mx:1010/1914 |
| Acesso em linha: | http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1914 |
| Access Level: | acceso abierto |
| Palavra-chave: | info:eu-repo/classification/Autor/Gold nanoparticles info:eu-repo/classification/Autor/Branced structures info:eu-repo/classification/Autor/Skin-equivalent phantoms info:eu-repo/classification/Autor/Dynamic infrared thermography info:eu-repo/classification/Autor/Protein conjugation info:eu-repo/classification/Autor/Cytotoxicity info:eu-repo/classification/cti/7 info:eu-repo/classification/cti/33 |
| Resumo: | "Anisotropic gold nanoparticles (AuNPs) are known as promising tools for localized photo-thermal therapy (PTT) of malignant cells. These anisotropic AuNPs present tunable surface plasmon resonances (SPR) with ideal NIR optical response to be applied as theranostic agents (nanoantennas systems). To this purpose, nanoparticles with branches are suitable because of the electromagnetic field concentrated at their vertices. In this work, we standardized a protocol to synthesize multibranched gold nanoparticles (MB-AuNPs) by the seed-growth method, and found size-seed dependence tunability on the hierarchy of branching. The optical response of MB-AuNPs was evaluated by UV-Vis spectroscopy, and their morphology by electronic microscopy (SEM and HRTEM). Once evaluated the optical response and monodispersity of AuNPs, we tested the MB-AuNPs immersed in skin-equivalent phantoms by dynamic infrared thermography (DIRT).1 The particle density of the synthesized colloids, has being determined by inducted coupled plasma spectrometry (ICP). Once a nano-synthesized system is being intended for biological purposes, it is necessary to carefully design the surface layer. In principle, the molecules at the surface of nanoparticles (NPs) will provide the signals to its long-term circulation, their biocompatibility and the selectivity to target cellular sites. Therefore, in this work and for intended medical applications of MB-AuNPs, a conjugated complex was prepared with bovine serum albumin (MB-AuNPs@BSA). The optical signals of the conjugate formation were evaluated by UV-Vis and fluorescence spectroscopy. The interaction of the protein with the surface of the MB-AuNPs was analyzed by Raman spectroscopy, to ensure the structure and function preservation of the protein. The full formation of a protein corona was determined by protein quantification in a microplates reader and later observed by HRTEM. The efficiency and stability of the conjugated was analyzed by Z-potential and through the interaction of the conjugated complex with cibacron blue. Toxicological test of bare MB-AuNPs and MB-AuNPs@BSA were performed in different cell lines. The models in-vitro were chosen according to main systems of indirect interaction of intravenously administered NPs. Cell viability and proliferation, mitochondrial activity and membrane integrity were analyzed." |
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