Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays

Hyperprolactinemia leads to several diagnoses, however,the immunoassay used for the prolactin determinationand the presence of macroprolactin, could induce a misdiagnosis.The aim of this study was to evaluate the usefulnessof separation and detection of macroprolactin by precipitationwith polyethyle...

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Autores: Jesús Rivas Espinosa, Ángel Trigos Landa, Virgilio Bocanegra García, Rosa Issel Acosta González, Anabel Bocanegra Alonso, Gildardo Rivera Sánchez
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:México
Institución:Universidad Autónoma de Tamaulipas
Repositorio:Redalyc-UAT
OAI Identifier:oai:redalyc.org:57631404
Acceso en línea:https://www.redalyc.org/articulo.oa?id=57631404
Access Level:acceso abierto
Palabra clave:Química
immunoassays
macroprolactin
hyperprolactinemia
Polyethylene glycol
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spelling Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassaysJesús Rivas EspinosaÁngel Trigos LandaVirgilio Bocanegra GarcíaRosa Issel Acosta GonzálezAnabel Bocanegra AlonsoGildardo Rivera SánchezQuímicaimmunoassaysmacroprolactinhyperprolactinemiaPolyethylene glycolHyperprolactinemia leads to several diagnoses, however,the immunoassay used for the prolactin determinationand the presence of macroprolactin, could induce a misdiagnosis.The aim of this study was to evaluate the usefulnessof separation and detection of macroprolactin by precipitationwith polyethylene glycol, using two commercialimmunoassays, Axsym Abbott and Advia Centaur Bayer inpatients with established diagnosis of hyperprolactinemia.One hundred and seventeen samples were tested. Sampleswere obtained from female patients aged between 22 to 59years old. We determined total prolactin (PRL), macroprolactinand free prolactin. In 30 of these samples hyperprolactinemiawas detected. PRL recovering percentages of supernatantfraction were from 6.5% to 78.5% and from26.2% to 75.4% when we compared Advia to Axsym systemsinitial values, respectively. Based on the PRL recoveringpercentage, 7 samples with significant macro-PRL presencewere observed. The macroprolactin prevalence in these30 samples, after PEG precipitation, was of 23% and 10%in the Axsym and Advia system respectively. Based uponthese findings, we establish that references values need tobe determined for both instruments, having applied thepolyethylene glycol precipitation for the prolactine determination.Asociación Mexicana de Bioquímica Clínica, A.C.2006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf0185-5751https://www.redalyc.org/articulo.oa?id=57631404Bioquimia (México) Num.4 Vol.31reponame:Redalyc-UATinstname:Universidad Autónoma de Tamaulipasinstacron:UATenhttp://www.redalyc.org/revista.oa?id=576Bioquimiainfo:eu-repo/semantics/openAccessoai:redalyc.org:576314042024-08-23T15:48:12Z
dc.title.none.fl_str_mv Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
title Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
spellingShingle Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
Jesús Rivas Espinosa
Química
immunoassays
macroprolactin
hyperprolactinemia
Polyethylene glycol
title_short Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
title_full Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
title_fullStr Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
title_full_unstemmed Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
title_sort Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
dc.creator.none.fl_str_mv Jesús Rivas Espinosa
Ángel Trigos Landa
Virgilio Bocanegra García
Rosa Issel Acosta González
Anabel Bocanegra Alonso
Gildardo Rivera Sánchez
author Jesús Rivas Espinosa
author_facet Jesús Rivas Espinosa
Ángel Trigos Landa
Virgilio Bocanegra García
Rosa Issel Acosta González
Anabel Bocanegra Alonso
Gildardo Rivera Sánchez
author_role author
author2 Ángel Trigos Landa
Virgilio Bocanegra García
Rosa Issel Acosta González
Anabel Bocanegra Alonso
Gildardo Rivera Sánchez
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Química
immunoassays
macroprolactin
hyperprolactinemia
Polyethylene glycol
topic Química
immunoassays
macroprolactin
hyperprolactinemia
Polyethylene glycol
description Hyperprolactinemia leads to several diagnoses, however,the immunoassay used for the prolactin determinationand the presence of macroprolactin, could induce a misdiagnosis.The aim of this study was to evaluate the usefulnessof separation and detection of macroprolactin by precipitationwith polyethylene glycol, using two commercialimmunoassays, Axsym Abbott and Advia Centaur Bayer inpatients with established diagnosis of hyperprolactinemia.One hundred and seventeen samples were tested. Sampleswere obtained from female patients aged between 22 to 59years old. We determined total prolactin (PRL), macroprolactinand free prolactin. In 30 of these samples hyperprolactinemiawas detected. PRL recovering percentages of supernatantfraction were from 6.5% to 78.5% and from26.2% to 75.4% when we compared Advia to Axsym systemsinitial values, respectively. Based on the PRL recoveringpercentage, 7 samples with significant macro-PRL presencewere observed. The macroprolactin prevalence in these30 samples, after PEG precipitation, was of 23% and 10%in the Axsym and Advia system respectively. Based uponthese findings, we establish that references values need tobe determined for both instruments, having applied thepolyethylene glycol precipitation for the prolactine determination.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0185-5751
https://www.redalyc.org/articulo.oa?id=57631404
identifier_str_mv 0185-5751
url https://www.redalyc.org/articulo.oa?id=57631404
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=576
dc.rights.none.fl_str_mv Bioquimia
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Bioquimia
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Asociación Mexicana de Bioquímica Clínica, A.C.
publisher.none.fl_str_mv Asociación Mexicana de Bioquímica Clínica, A.C.
dc.source.none.fl_str_mv Bioquimia (México) Num.4 Vol.31
reponame:Redalyc-UAT
instname:Universidad Autónoma de Tamaulipas
instacron:UAT
instname_str Universidad Autónoma de Tamaulipas
instacron_str UAT
institution UAT
reponame_str Redalyc-UAT
collection Redalyc-UAT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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