Purificación parcial de péptidos bioactivos con actividad antihipertensiva y antitrombótica de una leche comercial fermentada con Lactobacillus casei Shirota y Streptococcus thermophilus
The aim of this paper is to show that the fermented milk product by L. casei Shirota and Streptococcus thermophilus (Sofúl®) has peptides that are able to inhibit the fibrin clot formation by thrombin and/or to inhibit angiotensin converting enzyme (ACE). The commercial fermented product contains ag...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:9s161627n |
| Acceso en línea: | https://doi.org/10.24275/uami.9s161627n |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Probiotics -- Therapeutics info:eu-repo/classification/LEM/Lactobacillus casei Shirota info:eu-repo/classification/LEM/Streptococcus thermophilus info:eu-repo/classification/LEM/Microbial biotechnology info:eu-repo/classification/LEM/Prebiotics info:eu-repo/classification/LEM/Probióticos -- Uso terapéuticos info:eu-repo/classification/LEM/Prebióticos info:eu-repo/classification/cti/6 |
| Sumario: | The aim of this paper is to show that the fermented milk product by L. casei Shirota and Streptococcus thermophilus (Sofúl®) has peptides that are able to inhibit the fibrin clot formation by thrombin and/or to inhibit angiotensin converting enzyme (ACE). The commercial fermented product contains agar, carrageenan and gelatin, so a step of purification was necessary to prepare an extract free of gums. Eight peptides, which were purified by reversed phase and molecular exclusion chromatography, showed inhibitory activity of ACE and six showed antithrombotic activity. Those that demostrate the highest specific activity were tested under enzymatic digestion in vitro, although six peptides were not apparently modified by the gastric enzymes, only three of them retained its biological activity. Peptides 5 and 6 show the ACE inhibitory activity and antithrombotic. The peptide 5 had an inhibition efficiency ratio of ACE (IER) and antithrombotic activity of 21.3 % ± 1.6 % and 34.8 ± 14.3 respectively. Peptide 6 had an inhibition efficiency ratio ACE 24.6 % ± 0.1 and antithrombotic activity of 59.3 % ± 15.2. Furthermore, peptide 12 showed antithrombotic activity, which was retained after digestion with pepsin and trypsin 10.13 ± 33.82 %. This research is just one approach to multifunctional peptides whose bioactivities could affect positively the cardiovascular system through the consumption of fermented milk containing these peptides. |
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