PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTURE

We undertook this study to (1) evaluate an in vitro procedure for plantlet regeneration of Castilleja tenuiflora Benth. (Scrophulariaceae) from axillary buds and (2) inducecallugenesis and organogenesis through the manipulation of explant type, culturemedia and plant growth regulators. An efficient...

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Autores: Guadalupe Salcedo-Morales, Gabriel Rosas-Romero, Nayeli Nabor-Correa, Kalina Bermúdez-Torres, Alma R. López-Laredo, Gabriela Trejo-Tapia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:México
Institución:Centro de Investigación y de Estudios Avanzados del IPN
Repositorio:Redalyc-CINVESTAV
OAI Identifier:oai:redalyc.org:62112485006
Acceso en línea:https://www.redalyc.org/articulo.oa?id=62112485006
Access Level:acceso abierto
Palabra clave:Biología
organogenesis
Medicinal herb
micropropagation
Scrophulariaceae
shoot multiplication
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spelling PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTUREGuadalupe Salcedo-MoralesGabriel Rosas-RomeroNayeli Nabor-CorreaKalina Bermúdez-TorresAlma R. López-LaredoGabriela Trejo-TapiaBiologíaorganogenesisMedicinal herbmicropropagationScrophulariaceaeshoot multiplicationWe undertook this study to (1) evaluate an in vitro procedure for plantlet regeneration of Castilleja tenuiflora Benth. (Scrophulariaceae) from axillary buds and (2) inducecallugenesis and organogenesis through the manipulation of explant type, culturemedia and plant growth regulators. An efficient propagation protocol for in vitro multiplication and plantlet regeneration of C. tenuiflora using axillary buds of wild plants was developed. Shoot multiplication was induced from axillary buds in Murashige and Skoog (MS) medium containing 0.2 mg L-1 BAP and 0.1 mg L-1 NAA with an effi ciency of 33%. Shoot multiplication and elongation were achieved in one step using 0.1 mg L-1 IBA and 0.25 mg L-1 BAP. After 14 days, an average of four shoots per explant was observed. For rooting, IBA was increased to 1.0 mg L-1 and BAP was excluded. Hyperhydricity was not observed and 88% of the shoots rooted. From one axillary bud, 250 plantlets were produced within eight weeks. To induce callugenesis and organogenesis, explants (leaves and internodes) from plantlets were excisedand inoculated into MS, B5 and NN culture media in combination with NAA (0-10 uM) and kinetin (0-0.5 uM). In general, rhizogenesis was the main in vitro response (up to 100%) followed by shoot formation (5-50%) and, finally, callugenesis (2-35%). Internodes were more competent than leaves for both callugenesis and organogenesis, along with the fact that leaf explants oxidized easily. Rhizogenesis depended on exogenous NAA, but auxin requirement varied according to the culture medium and type of explant used. On the basis of our results, conditions for callugenesis and organogenesis induction of C. tenuiflora can be recommended: a) callus-internode, 0.1 uM NAA and B5 medium; b) rhizogenesis- 0.1 uM NAA and NN medium; and c) shoots-internode, 0.1 ¿M NAA and MS medium. Results of the present study show the feasibility of using in vitro culture to propagate and conserve germplasm of the -'ancer herb' C. tenuiflora.Instituto Politécnico Nacional2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf1405-2768https://www.redalyc.org/articulo.oa?id=62112485006Polibotánica (México) Num.28reponame:Redalyc-CINVESTAVinstname:Centro de Investigación y de Estudios Avanzados del IPNinstacron:CINVESTAVenhttp://www.redalyc.org/revista.oa?id=621Polibotánicainfo:eu-repo/semantics/openAccessoai:redalyc.org:621124850062024-08-23T16:11:44Z
dc.title.none.fl_str_mv PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTURE
title PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTURE
spellingShingle PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTURE
Guadalupe Salcedo-Morales
Biología
organogenesis
Medicinal herb
micropropagation
Scrophulariaceae
shoot multiplication
title_short PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTURE
title_full PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTURE
title_fullStr PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTURE
title_full_unstemmed PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTURE
title_sort PROPAGATION AND CONSERVATION OF CASTILLEJA TENUIFLORA BENTH. ("HIERBA DEL CANCER") THROUGH IN VITRO CULTURE
dc.creator.none.fl_str_mv Guadalupe Salcedo-Morales
Gabriel Rosas-Romero
Nayeli Nabor-Correa
Kalina Bermúdez-Torres
Alma R. López-Laredo
Gabriela Trejo-Tapia
author Guadalupe Salcedo-Morales
author_facet Guadalupe Salcedo-Morales
Gabriel Rosas-Romero
Nayeli Nabor-Correa
Kalina Bermúdez-Torres
Alma R. López-Laredo
Gabriela Trejo-Tapia
author_role author
author2 Gabriel Rosas-Romero
Nayeli Nabor-Correa
Kalina Bermúdez-Torres
Alma R. López-Laredo
Gabriela Trejo-Tapia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología
organogenesis
Medicinal herb
micropropagation
Scrophulariaceae
shoot multiplication
topic Biología
organogenesis
Medicinal herb
micropropagation
Scrophulariaceae
shoot multiplication
description We undertook this study to (1) evaluate an in vitro procedure for plantlet regeneration of Castilleja tenuiflora Benth. (Scrophulariaceae) from axillary buds and (2) inducecallugenesis and organogenesis through the manipulation of explant type, culturemedia and plant growth regulators. An efficient propagation protocol for in vitro multiplication and plantlet regeneration of C. tenuiflora using axillary buds of wild plants was developed. Shoot multiplication was induced from axillary buds in Murashige and Skoog (MS) medium containing 0.2 mg L-1 BAP and 0.1 mg L-1 NAA with an effi ciency of 33%. Shoot multiplication and elongation were achieved in one step using 0.1 mg L-1 IBA and 0.25 mg L-1 BAP. After 14 days, an average of four shoots per explant was observed. For rooting, IBA was increased to 1.0 mg L-1 and BAP was excluded. Hyperhydricity was not observed and 88% of the shoots rooted. From one axillary bud, 250 plantlets were produced within eight weeks. To induce callugenesis and organogenesis, explants (leaves and internodes) from plantlets were excisedand inoculated into MS, B5 and NN culture media in combination with NAA (0-10 uM) and kinetin (0-0.5 uM). In general, rhizogenesis was the main in vitro response (up to 100%) followed by shoot formation (5-50%) and, finally, callugenesis (2-35%). Internodes were more competent than leaves for both callugenesis and organogenesis, along with the fact that leaf explants oxidized easily. Rhizogenesis depended on exogenous NAA, but auxin requirement varied according to the culture medium and type of explant used. On the basis of our results, conditions for callugenesis and organogenesis induction of C. tenuiflora can be recommended: a) callus-internode, 0.1 uM NAA and B5 medium; b) rhizogenesis- 0.1 uM NAA and NN medium; and c) shoots-internode, 0.1 ¿M NAA and MS medium. Results of the present study show the feasibility of using in vitro culture to propagate and conserve germplasm of the -'ancer herb' C. tenuiflora.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1405-2768
https://www.redalyc.org/articulo.oa?id=62112485006
identifier_str_mv 1405-2768
url https://www.redalyc.org/articulo.oa?id=62112485006
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=621
dc.rights.none.fl_str_mv Polibotánica
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Polibotánica
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Politécnico Nacional
publisher.none.fl_str_mv Instituto Politécnico Nacional
dc.source.none.fl_str_mv Polibotánica (México) Num.28
reponame:Redalyc-CINVESTAV
instname:Centro de Investigación y de Estudios Avanzados del IPN
instacron:CINVESTAV
instname_str Centro de Investigación y de Estudios Avanzados del IPN
instacron_str CINVESTAV
institution CINVESTAV
reponame_str Redalyc-CINVESTAV
collection Redalyc-CINVESTAV
repository.name.fl_str_mv
repository.mail.fl_str_mv
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