Efficient Micropropagation of Sedum sediforme and S. album for Large-Scale Propagation and Integration into Green Roof Systems

[EN] Urban expansion has led to two significant environmental challenges: the reduction in green spaces and the rise in urban temperatures, decreasing city livability. Green roofs have emerged as a sustainable solution to mitigate these issues, offering ecological and economic benefits while improvi...

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Bibliographic Details
Authors: Moreno-García, Ignacio|||0000-0001-5874-4297, Soler Aleixandre, Salvador|||0000-0001-8325-3326, Rodríguez Burruezo, Adrián|||0000-0002-4530-8071, Moreno Ferrero, Vicente|||0000-0002-0345-7300, Pineda Chaza, Benito José|||0000-0001-9176-701X, García Sogo, Begoña
Format: article
Publication Date:2025
Country:España
Institution:Universitat Politècnica de València (UPV)
Repository:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Language:English
OAI Identifier:oai:riunet.upv.es:10251/225311
Online Access:https://riunet.upv.es/handle/10251/225311
Access Level:Open access
Keyword:Micropropagation
Axillary shoot culture
Somatic organogenesis
Crassulaceae
Description
Summary:[EN] Urban expansion has led to two significant environmental challenges: the reduction in green spaces and the rise in urban temperatures, decreasing city livability. Green roofs have emerged as a sustainable solution to mitigate these issues, offering ecological and economic benefits while improving building energy efficiency. Some species of the genus Sedum, particularly Sedum sediforme and Sedum album, are ideal for such green infrastructure due to their non-aggressive and superficial root system, high drought tolerance, low nutrient needs, pest and disease resistance, and metabolic adaptability during dry periods. This study aims to optimize the large-scale production of two native ecotypes of S. sediforme and S. album from the Valencian Community through an efficient propagation system that enables uniform plant production in limited space. For this purpose, we have developed micropropagation systems that allow a rapid multiplication of these two species. A direct morphogenesis system was established using axenic plant shoots, and a protocol for adventitious organogenesis from leaves was also developed. These methods significantly enhance propagation speed, spatial efficiency, and plant uniformity. Notably, the metabolic plasticity of S. sediforme and S. album reduces abiotic stress during acclimatization, promoting efficient ex vitro establishment and functional integration into extensive green roof ecosystems.