Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg.

Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-Glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all in...

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Detalhes bibliográficos
Autores: JESUS ALEJANDRO YAM PUC, ELIDE AVILES BERZUNZA, MANUEL JESUS CHAN BACAB, LUIS MANUEL PEÑA RODRIGUEZ, GREGORIO DEL CARMEN GODOY HERNANDEZ
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2012
País:México
Recursos:Centro de Investigación Científica de Yucatán
Repositório:Repositorio Institucional CICY
Idioma:inglês
OAI Identifier:oai:cicy.repositorioinstitucional.mx:1003/730
Acesso em linha:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/730
Access Level:Acceso aberto
Palavra-chave:info:eu-repo/classification/Autores/ Β-GLUCURONIDASE
info:eu-repo/classification/Autores/AGROBACTERIUM TUMEFACIENS
info:eu-repo/classification/Autores/KANAMYCIN
info:eu-repo/classification/Autores/APOCYNACEAE
info:eu-repo/classification/cti/2
Descrição
Resumo:Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-Glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our knowledge, this is the first report of Pentalinon andrieuxii susceptibility to Agrobacterium tumefaciens-mediated genetic transformation.

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