Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed tr...

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Detalhes bibliográficos
Autores: GREGORIO DEL CARMEN GODOY HERNANDEZ, ELIDE AVILES BERZUNZA, MILDRED RUBI CARRILLO PECH, FELIPE AUGUSTO VAZQUEZ FLOTA
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:México
Recursos:Centro de Investigación Científica de Yucatán
Repositorio:Repositorio Institucional CICY
Idioma:inglés
OAI Identifier:oai:cicy.repositorioinstitucional.mx:1003/785
Acesso em linha:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/785
Access Level:acceso abierto
Palavra-chave:info:eu-repo/classification/Autores/Β-GLUCURONIDASE
info:eu-repo/classification/Autores/ARGEMONE MEXICANA
info:eu-repo/classification/Autores/BENZYLISOQUINOLINE ALKALOIDS
info:eu-repo/classification/Autores/GENETIC TRANSFORMATION
info:eu-repo/classification/Autores/NEOMYCIN PHOSPHOTRANSFERASE II
info:eu-repo/classification/cti/2
Descrição
Resumo:Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation.