Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed tr...
| Autores: | , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2008 |
| País: | México |
| Recursos: | Centro de Investigación Científica de Yucatán |
| Repositorio: | Repositorio Institucional CICY |
| Idioma: | inglés |
| OAI Identifier: | oai:cicy.repositorioinstitucional.mx:1003/785 |
| Acesso em linha: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/785 |
| Access Level: | acceso abierto |
| Palavra-chave: | info:eu-repo/classification/Autores/Β-GLUCURONIDASE info:eu-repo/classification/Autores/ARGEMONE MEXICANA info:eu-repo/classification/Autores/BENZYLISOQUINOLINE ALKALOIDS info:eu-repo/classification/Autores/GENETIC TRANSFORMATION info:eu-repo/classification/Autores/NEOMYCIN PHOSPHOTRANSFERASE II info:eu-repo/classification/cti/2 |
| Resumo: | Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation. |
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