Estudio del polimorfismo de los genes de los receptores tipo Toll 2, 4, 5 y 9 y su asociación con enfermedades causadas por Helicobacter pylori en dos poblaciones latinoamericanas
Helicobacter pylori (Hp) is a Gram negative bacillus that infects 80% of the world population. Hp infection activates both the innate and the adaptive immune response and is associated with different gastroduodenal diseases. However, only 30% of the infected population develops the some of the assoc...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:b8515n50j |
| Acceso en línea: | https://doi.org/10.24275/uami.b8515n50j |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Human genetics -- Variation info:eu-repo/classification/LEM/Polimorfismos genéticos info:eu-repo/classification/LEM/Biología experimental info:eu-repo/classification/LEM/Research info:eu-repo/classification/LEM/Helicobacter pylori -- Investigación info:eu-repo/classification/LEM/Genética humana -- Variación info:eu-repo/classification/LEM/Genetic polymorphisms info:eu-repo/classification/LEM/Biology, Experimental info:eu-repo/classification/cti/3 |
| Sumario: | Helicobacter pylori (Hp) is a Gram negative bacillus that infects 80% of the world population. Hp infection activates both the innate and the adaptive immune response and is associated with different gastroduodenal diseases. However, only 30% of the infected population develops the some of the associated pathology. Toll-like receptors (TLRs) are receptors of the innate immune system that are able to recognize Hp and activate and induce intracellular signals that in turn activates inflammatory genes like IL-1, TNF-α, IL-6 and IL-8. Polymorphisms D299G and T399I in TLR4 and R753Q in TLR2 have been associated with an increased risk of developing infections. Similarly, Arg392stop and N592S in TLR5 are associated with an increased risk of infection and a predisposition to develop autoimmune diseases. The polymorphism in the promoter region of TLR9 gene -1237T/C has also been associated with autoimmune diseases as well as gastroduodenal diseases. Because these studies have been conducted in Caucasians and don’t known polymorphic variations in Latin American population, the objective of these study was to evaluate the presence of these polymorphisms in two Latin American populations and their association to pathologies caused by Hp. Real-time PCR was used to determine allelic discrimination between the polymorphisms above mentioned in samples of a Paraguayan and Colombian population. Patients were grouped according to the clinical diagnosis: non-atrophic gastritis, atrophic gastritis, metaplasia, cancer and duodenal ulcer; taking as a control group patients with non-atrophic gastritis. We found that neither of the two populations studied had statistically significant differences in the frequency of polymorphisms in TLR 2, 4 and 5 nor association with the pathologies studied. However, we observed an increase in the frequency of the TLR9 -1237T/C polymorphism in the Paraguayan population with a a statistically significant difference (p=0.04) in the group of duodenal ulcer with an OR of 3.7. These results suggest that the -1237T/C polymorphism in TLR9 is a risk factor for developing duodenal ulcer in the Paraguayan population and would require a larger number of samples to conclude whether such polymorphism is similarly important in Colombians. |
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