Análisis espectral de nanopartículas plasmónicas usando microscopía de campo obscuro
In this work, we present a dark field microscope for the study of gold nanoparticles (NPs). This microscopy method is based on a configuration of total internal reflection (TIR) that allows to obtain images of NPs excited by an evanescent field. Our system is based on an objetive of a large numerica...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2019 |
| País: | México |
| Institución: | Instituto Nacional de Astrofísica, Óptica y Electrónica |
| Repositorio: | Repositorio Institucional del INAOE |
| Idioma: | español |
| OAI Identifier: | oai:inaoe.repositorioinstitucional.mx:1009/2011 |
| Acceso en línea: | http://inaoe.repositorioinstitucional.mx/jspui/handle/1009/2011 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/Inspec/Nanobiophotonics info:eu-repo/classification/Inspec/Single-molecule info:eu-repo/classification/Inspec/Plasmonic nanoparticles info:eu-repo/classification/Inspec/Dark field microscopy info:eu-repo/classification/cti/1 info:eu-repo/classification/cti/22 info:eu-repo/classification/cti/2209 info:eu-repo/classification/cti/220990 |
| Sumario: | In this work, we present a dark field microscope for the study of gold nanoparticles (NPs). This microscopy method is based on a configuration of total internal reflection (TIR) that allows to obtain images of NPs excited by an evanescent field. Our system is based on an objetive of a large numerical aperture (NA), which collects light and sends it to a CMOS detector used as an eyepiece. We developed a light collection system, which allows scattered light to be collected by the gold NPs through a multimode optical fiber coupled to the dark field microscope. This light is finally transmitted and analyzed by a spectrometer. Two samples of gold nanoparticles were analyzed. A sample in solution, chemically synthesized by the Turkevich method and a sample made by a lithographic method which has gold nanostructures printed on glass. A lighting system based on total internal reflection was implemented, which uses two light emitting diodes (LED) and a slide used as a waveguide, an economical, novel and less difficult to use system. The results show that our dark field microscope is a method that distinguishes gold nanoparticles with diameters of 26 nm and 39 nm. It was also possible to simultaneously measure the spectrum scattered by said gold nanoparticles and the changes in their surrounding environment. |
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