High-frequency reversion of geminivirus replication protein mutants during infection

"The geminivirus replication protein AL1 interacts with retinoblastoma-related protein (RBR), a key regulator of the plant division cell cycle, to induce conditions permissive for viral DNA replication. Previous studies of tomato golden mosaic virus (TGMV) AL1 showed that amino acid L148 in the...

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Autores: GERARDO RAFAEL ARGUELLO ASTORGA, JOSE TRINIDAD ASCENCIO IBAÑEZ
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:México
Institución:Instituto Potosino de Investigación Científica y Tecnológica
Repositorio:Repositorio Institucional del IPICYT
Idioma:inglés
OAI Identifier:oai:ipicyt.repositorioinstitucional.mx:1010/1086
Acceso en línea:http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1086
Access Level:acceso embargado
Palabra clave:info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2420
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spelling High-frequency reversion of geminivirus replication protein mutants during infectionGERARDO RAFAEL ARGUELLO ASTORGAJOSE TRINIDAD ASCENCIO IBAÑEZinfo:eu-repo/classification/cti/2info:eu-repo/classification/cti/24info:eu-repo/classification/cti/2420info:eu-repo/classification/cti/2420"The geminivirus replication protein AL1 interacts with retinoblastoma-related protein (RBR), a key regulator of the plant division cell cycle, to induce conditions permissive for viral DNA replication. Previous studies of tomato golden mosaic virus (TGMV) AL1 showed that amino acid L148 in the conserved helix 4 motif is critical for RBR binding. In this work, we examined the effect of an L148V mutation on TGMV replication in tobacco cells and during infection of Nicotiana benthamiana plants. The L148V mutant replicated 100 times less efficiently than wild-type TGMV in protoplasts but produced severe symptoms that were delayed compared to those of wild-type infection in plants. Analysis of progeny viruses revealed that the L148V mutation reverted at 100% frequency in planta to methionine, leucine, isoleucine, or a second-site mutation depending on the valine codon in the initial DNA sequence. Similar results were seen with another geminivirus, cabbage leaf curl virus (CaLCuV), carrying an L145A mutation in the equivalent residue. Valine was the predominant amino acid recovered from N. benthamiana plants inoculated with the CaLCuV L145A mutant, while threonine was the major residue in Arabidopsis thaliana plants. Together, these data demonstrated that there is strong selection for reversion of the TGMV L148V and CaLCuV L145A mutations but that the nature of the selected revertants is influenced by both the viral background and host components. These data also suggested that high mutation rates contribute to the rapid evolution of geminivirus genomes in plants."American Society for Microbiology2007-10info:eu-repo/date/embargoEnd/2021-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1086reponame:Repositorio Institucional del IPICYTinstname:Instituto Potosino de Investigación Científica y Tecnológicainstacron:IPICYTenginfo:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1128/JVI.00925-07info:eu-repo/semantics/embargoedAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:ipicyt.repositorioinstitucional.mx:1010/10862024-08-28T03:17:35Z
dc.title.none.fl_str_mv High-frequency reversion of geminivirus replication protein mutants during infection
title High-frequency reversion of geminivirus replication protein mutants during infection
spellingShingle High-frequency reversion of geminivirus replication protein mutants during infection
GERARDO RAFAEL ARGUELLO ASTORGA
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2420
info:eu-repo/classification/cti/2420
title_short High-frequency reversion of geminivirus replication protein mutants during infection
title_full High-frequency reversion of geminivirus replication protein mutants during infection
title_fullStr High-frequency reversion of geminivirus replication protein mutants during infection
title_full_unstemmed High-frequency reversion of geminivirus replication protein mutants during infection
title_sort High-frequency reversion of geminivirus replication protein mutants during infection
dc.creator.none.fl_str_mv GERARDO RAFAEL ARGUELLO ASTORGA
JOSE TRINIDAD ASCENCIO IBAÑEZ
author GERARDO RAFAEL ARGUELLO ASTORGA
author_facet GERARDO RAFAEL ARGUELLO ASTORGA
JOSE TRINIDAD ASCENCIO IBAÑEZ
author_role author
author2 JOSE TRINIDAD ASCENCIO IBAÑEZ
author2_role author
dc.subject.none.fl_str_mv info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2420
info:eu-repo/classification/cti/2420
topic info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2420
info:eu-repo/classification/cti/2420
description "The geminivirus replication protein AL1 interacts with retinoblastoma-related protein (RBR), a key regulator of the plant division cell cycle, to induce conditions permissive for viral DNA replication. Previous studies of tomato golden mosaic virus (TGMV) AL1 showed that amino acid L148 in the conserved helix 4 motif is critical for RBR binding. In this work, we examined the effect of an L148V mutation on TGMV replication in tobacco cells and during infection of Nicotiana benthamiana plants. The L148V mutant replicated 100 times less efficiently than wild-type TGMV in protoplasts but produced severe symptoms that were delayed compared to those of wild-type infection in plants. Analysis of progeny viruses revealed that the L148V mutation reverted at 100% frequency in planta to methionine, leucine, isoleucine, or a second-site mutation depending on the valine codon in the initial DNA sequence. Similar results were seen with another geminivirus, cabbage leaf curl virus (CaLCuV), carrying an L145A mutation in the equivalent residue. Valine was the predominant amino acid recovered from N. benthamiana plants inoculated with the CaLCuV L145A mutant, while threonine was the major residue in Arabidopsis thaliana plants. Together, these data demonstrated that there is strong selection for reversion of the TGMV L148V and CaLCuV L145A mutations but that the nature of the selected revertants is influenced by both the viral background and host components. These data also suggested that high mutation rates contribute to the rapid evolution of geminivirus genomes in plants."
publishDate 2007
dc.date.none.fl_str_mv 2007-10
info:eu-repo/date/embargoEnd/2021-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1086
url http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1086
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1128/JVI.00925-07
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositorio Institucional del IPICYT
instname:Instituto Potosino de Investigación Científica y Tecnológica
instacron:IPICYT
instname_str Instituto Potosino de Investigación Científica y Tecnológica
instacron_str IPICYT
institution IPICYT
reponame_str Repositorio Institucional del IPICYT
collection Repositorio Institucional del IPICYT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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